CLONING, SEQUENCING, EXPRESSION AND CHROMOSOMAL LOCALIZATION OF HUMAN ERG GENE

复制缺陷型禽成红细胞增多症病毒E26， 在鸡中诱导混合型红细胞/髓细胞白血病。 E26 包括来自两个原癌基因的元素，鸡原myb 和鸡的原Ets，以及病毒gag基因的Δ gag。 克隆了与ets区域同源的人类基因组克隆 并且通过部分序列显示与V-ETS区域相关 分析. 人类11号染色体上的ets-1基因座编码一种 单个6.8kb的mRNA;人ets-2基因座编码三个 4.7kb、3.2kb和2.7kb的mRNA。 Hu-ets-1和Hu-ets-2 基因最近被证明在某些 白血病 由于ets在肿瘤形成中的重要性，我们 开始寻找其他与人类基因密切相关的基因， ets. 从人科洛320细胞制备cDNA文库 表达非常高水平的ETS特异性转录物的细胞系。 分离出两个与Hu-ets-2探针反应的cDNA克隆。 通过限制性酶切图谱表征这些克隆， 序列分析表明，它们代表了完整的 一个新的人类基因erg（ets-related）的编码序列 基因）。 erg基因显示出约40%的同源性， 约70%的蛋白质被转移到该蛋白质的5'和3'区的两个结构域。 v-ets癌基因 其中一个cDNA（erg-2）与erg-1的差异在于： 导致NH-2附近编码移码的剪接事件 末端，导致在氨基处另外的99 a-a插入 终端 有初步证据表明erg-2 可以使用不同的翻译起始和多聚腺苷酸化 信号了 全长cDNA克隆erg-1和erg-2， 在E.大肠杆菌和哺乳动物细胞中。 erg基因定位于人类21号染色体上。 原位 erg基因染色体定位的杂交研究 正在进行中。 因此，erg基因的精确位置和 分析erg基因座在不同的人类癌症，唐氏 综合症和阿尔茨海默病应该可以 确定是否扩增，易位或重排这一点， 基因可能与这些疾病中的任何一种有关。

The replication-defective avian erythroblastosis virus, E26, induces a mixed erythroid/myeloid leukemia in chickens. E26 includes elements from two proto-oncogenes, chicken proto-myb and chicken proto-ets, and delta gag from the viral gag gene. Human genomic clones homologous to the ets region were cloned and shown to be related to the v-ets region by partial sequence analysis. The human ets-1 locus on chromosome 11 encodes a single mRNA of 6.8 kb; the human ets-2 locus encodes three mRNAs of 4.7 kb, 3.2 kb and 2.7 kb. The Hu-ets-1 and Hu-ets-2 genes have recently been shown to be transposed in certain leukemias. Because of the significance of ets in neoplasia, we embarked on a search for other human genes closely related to ets. A cDNA library was prepared from a human COLO 320 cell line which expresses very high levels of ets-specific transcripts. Two cDNA clones reactive with the Hu-ets-2 probe were isolated. Characterization of these clones by restriction mapping and sequence analysis revealed that they represented the complete coding sequence of a novel human gene named erg (ets-related gene). The erg gene shows a homology of approximately 40% and approximately 70% to two domains of the 5' and 3' regions of the v-ets oncogene. One of the cDNAs (erg-2) differs from erg-1 by a splicing event that causes a coding frameshift near the NH-2 terminus, resulting in an additional 99 a-a insertion at the amino terminal end. There is preliminary evidence to state that erg-2 may use a different translation initiation and poly-adenylation signal. The full-length cDNA clones, erg-1 and erg-2, are being expressed in vitro and in vivo in E. coli and in mammalian cells. The erg gene has been localized on human chromosome 21. In situ hybridization studies for chromosomal localization of the erg gene are in progress. Thus, the precise location of the erg gene and analysis of the erg locus in different human cancers, Down's syndrome and Alzheimer's disease should make it possible to determine if amplification, translocation or rearrangement of this gene can be linked to any of these diseases.