QUANTITATIVE STUDIES ON CONCURRENT FACTORS IN NEOPLASTIC TRANSFORMATION

对小鼠转化机制的研究仍在继续 胚胎细胞系，BALB/3T3克隆A31-1-1。转型是 用几种金属得到的。三价和三价的研究 五价砷(As)表明，三价As是四倍 比五价砷更活跃。但是，当与 计算为摄取和细胞负荷、毒性和转化 发现两种形式的AS的频率是相同的。这个 代谢研究结果表明，三价砷是形式 对有毒和转化的影响负责，独立负责 砷暴露的价态。 金属和有机致癌物转化的BALB/3T3细胞， 或自发地，当接种到裸鼠身上时，发现 致癌但不转移，即使通过静脉注射也是如此 行政管理。这一发现与缺乏类型是一致的 转化细胞中观察到的IV型胶原溶解活性 台词。通过进一步治疗来诱导转移活性 Ras癌基因或与致癌物的关系正在调查中。

Studies were continued on mechanisms of transformation in the mouse embryo cell line, BALB/3T3 clone A31-1-1. Transformation was obtained with several metals. Studies with trivalent and pentavalent arsenic (As) showed that trivalent As was four times more active than pentavalent As. However, when correlated with the calculated As uptake and cell burden, toxicity and transformation frequency were found to be the same for both forms of As. The results of metabolic studies indicate that trivalent As is the form responsible for the toxic and transforming effects, independently of the valence state of the As exposure. BALB/3T3 cells transformed by metals and by organic carcinogens, or spontaneously, when inoculated in nude mice were found to be tumorigenic but not metastasizing, even by intravenous administration. This finding is consistent with the lack of type IV collagenolytic activity observed in these transformed cell lines. Induction of metastatic activity by further treatment with the ras oncogene or with carcinogens is under investigation.