CHARACTERIZATION OF THE GENE PRODUCTS OF THE ERG (ETS-RELATED GENE) LOCUS

Erq(Ets-Ets)编码的Erq蛋白的存在。 相关基因)位点已经在特定的人类细胞系中被检测到。 为了探索erq蛋白，制备了多克隆抗血清。 对抗代表氨基酸序列的合成多肽 从erg基因位点的DNA序列中推断出来。此外， 我们必须促进对推测的ERG的进一步分析 我们已经纯化了一种细菌表达的erg蛋白，并 正在使用这种高度纯化的蛋白质来培养多克隆erg 抗血清。在来自核组分的免疫沉淀物中 35S-蛋氨酸标记的COLO 320细胞检测到两种56蛋白 与特异性ERG-RI特异反应的63kD 抗血清。这些蛋白质是否代表细胞erq基因 产品目前正在接受调查。

The presence of putative erq proteins encoded by the erq (ets- related gene) locus has been examined in specific human cell lines. To probe the erq proteins, polyclonal antisera have been prepared against synthetic polypeptides representing amino acid sequences deduced from the DNA sequence of the erg gene locus. Furthermore, we have to facilitate further analysis of the putative erg proteins; we have purified a bacterially expressed erg protein and are using this highly purified protein to raise polyclonal erg antiserum. In immunoprecipitates from the nuclear fractions of 35S-methionine-labeled COLO 320 cells we detect two proteins of 56 and 63 Kd which reacted specifically with the specific erg-RI antiserum. Whether these proteins represent the cellular erq gene products is currently under investigation.