BIOSYNTHESIS AND GLYCOSYLATION OF THYROTROPIN

碳水化合物在大多数糖蛋白中的作用尚不清楚。 此外，人们对内分泌和 碳水化合物结构的发育调控。使用新的 开发的高效液相色谱方法，我们 已表明来自甲状腺功能低下啮齿类动物的促甲状腺激素(TSH)具有更多 唾液酸化和较少的硫酸盐化链。同样，在发展中 唾液酸化的增加以及更复杂的 碳水化合物链。这样的变化与 代谢清除率和体内推定增加 生物活性。碳水化合物加工的抑制剂最终进入决赛 复杂的糖结构影响TSH的分泌和分选。 这些研究表明，糖蛋白的碳水化合物加工 可能受到调控，并影响多种生物学功能。

The role of carbohydrates in most glycoproteins is not known. Moreover, there is little known about the endocrine and developmental regulation of carbohydrate structure. Using newly developed methods of high performance liquid chromatography, we have shown that thyrotropin (TSH) from hypothyroid rodents has more sialylated and less sulfated chains. Similarly, in development there is an increase in sialylation as well as more complex carbohydrate chains. Such changes correlate with a delay in metabolic clearance rate and presumably increased in vivo bioactivity. Inhibitors of carbohydrate processing to final complex sugar structures affect the secretion and sorting of TSH. These studies suggest that carbohydrate processing of glycoproteins may be regulated and affect multiple biological functions.