TRANSPLANT OF GABAERGIC NEURONS INTO THE SUBSTANTIA NIGRA OF KINDLED RATS

该项目将研究神经移植的效果。 从16日龄大鼠胚胎的小脑悬液进入 成年点燃大鼠的黑质。我们的假设是 黑质参与控制癫痫发作的扩散 并加入γ-氨基丁酸(GABA)能神经元 到黑质就能防止癫痫发作。 GABA能神经元是通过温和的胰酶消化获得的 16日龄胚胎的小脑。然后这些细胞就会 用哈佛泵移植到黑质内。这个 细胞的来源将通过染色确定 谷氨酸脱羧酶抗体与小脑 特定的抗体。 我们的最新结果表明，在光镜水平上， 黑质移植区的细胞网络。这些 细胞呈拉长状，突起广泛。他们有 GAD抗体染色失败，但其他GABA能神经元 该地区也没有被污染。我们目前正在调查 这背后的原因。初步工作表明，点燃 移植后消失2周，但随后又回来了。这个 这些发现的原因正在调查中。 黑质已被证明是一个控制点 多名调查人员进行了查封。目前的研究将 试图证明GABA能神经元移植 来自大鼠胚胎的小脑会阻止癫痫的蔓延。

This project will investigate the effect of transplanting neuronal suspensions from the cerebellum of 16 day old rat embryos into the substantia nigra of adult kindled rats. The hypothesis is that the substantia nigra is involved in the control of spread of seizures and the addition of gamma amino butyric acid (GABA)ergic neurons to the nigra will prevent the seizures. GABA ergic neurons are obtained by gentle trypsinization of the cerebellum of 16 days old embryos. These cells are then transplanted into the substania nigra using a Harvard pump. The origin of the cells will be determined by staining with the glutamate decarboxylase (GAD) antibody as well as cerebellum specific antibodies. Our most recent results show at a light microscopic level a fine network of cells at the area of transplant in the nigra. These cells are elongated in shape with extensive processes. They have failed to stain with GAD antibody, but other GABA ergic neurons in the area have not stained either. We are currently investigating the reasons behind this. Preliminary work has shown that kindling disappears for 2 weeks after transplant but then returns. The reasons for these findings are being investigated. The substantia nigra has been shown to be a controlling point of seizure spread by numerous investigators. The current study will attempt to demonstrate whether transplants of GABA ergic neurons from rat embryo cerebellum will block the spread of seizures.