BIOSYNTHESIS AND GLYCOSYLATION OF THYROTROPIN

糖蛋白是最常见的一类病毒和 原虫包膜蛋白，哺乳动物血清蛋白，血浆 膜受体、转运蛋白和离子通道。 虽然 已经表明，这些蛋白质可能具有异质性， 碳水化合物的结构，具体的化学性质和 这种异质性的重要性尚不清楚。 使用新 开发了糖肽凝集素分析方法， 高效液相色谱（HPLC）分析 酶释放的糖，我们已经阐明了至少9种不同的 分泌型TSH的碳水化合物变体。 此外，对于第一个 时间在任何糖蛋白系统，我们已经阐明了主要 这种糖基化的发育和内分泌调节，和 目前正在研究这种调节的功能相关性， 异质性 这些研究可能揭示了一个新的后- 翻译控制机制可以解释 甲状腺生理学和病理生理学。

Glycoproteins constitute the most common class of viral and protozoal envelope proteins, mammalian serum proteins, plasma membrane receptors, transporters and ion channels. Although it has been suggested that such proteins may have heterogeneity of carbohydrate structures, the specific chemical nature and significance of such heterogeneity is not known. Using newly developed methods of lectin analysis of glycopeptides as well as high performance liquid chromatography (HPLC) analysis of enzyme-released sugars, we have elucidated at least 9 different carbohydrate variants of secreted TSH. Moreover, for the first time in any glycoprotein system we have elucidated major developmental and endocrine regulation of such glycosylation, and are currently studying the functional correlates of such regulated heterogeneity. These studies may reveal a novel post- translational control mechanism that may explain various aspects of thyroid physiology and pathophysiology.