EFFECT OF DIETARY RESTRICTION ON GENE EXPRESSION

这项研究的基本目标是确定饮食对 限制了遗传信息在整个生命周期中的转移 有机体的。在这项研究中，雄性Fischer F344大鼠将在实验室喂养 自由饮食或60%的自由饮食(限制饮食)。中庸之道 这两组大鼠的最大存活率将被确定。 周期性地，翻译和转录活动的几个 将测定两种饮食方案下大鼠的组织。使用 脑和肾组织线粒体后上清液的分离 肝细胞，蛋白质合成将通过掺入 放射性标记L-缬氨酸制成酸不溶物质的测定 本研究中大鼠这些组织的翻译活性。这个 肝脏的转录活性将通过测量RNA合成来确定 通过肝细胞悬液结合的能力 放射性标记的奥罗里酸不溶物质。从这项研究来看，它 将有可能确定延长的寿命是否与 饮食限制伴随着RNA或蛋白质合成的减少 蛋白质和/或与年龄相关的RNA或 蛋白。

The basic objective of this study is to determine what effect dietary restriction has on the transfer of genetic information throughout the life span of an organism. In this study, male Fischer F 344 rats will be fed a laboratory chow diet ad libitum or sixty percent of ad libitum (restricted diet). The mean and maximum survival of these two groups of rats will be established. Periodically, the translational and transcriptional activities of several tissues from rats on the two dietary regimens will be determined. Using the post-mitochondrial supernatant from brain and kidney tissue and isolated hepatocytes, protein synthesis will be measured by the incorporation of radioactively-labeled L-valine into acid-insoluble material to determine the translational activities of these tissues from rats in this study. The transcriptioal activity of liver will be ascertained by measuring RNA synthesis by the ability of suspensions of hepatocytes to incorporate radioactively-labeled orotic acid-insoluble material. From this research, it will be possible to determine whether the increased longevity associated with dietary restriction is accompanied by a decrease in the synthesis of RNA or protein and/or a delay in the age-related changes in the synthesis of RNA or protein.