MONOCLONAL ANTIBODIES IN THE DIAGNOSIS OF PNEUMOCYSTIS PNEUMONIA

肺孢子虫感染的诊断方法 卡氏虫（PC）依赖于具有典型形态的生物体的展示 使用特殊染料，如次甲基胺银、甲苯胺蓝O等。 这些污点都不是特异性的，它们都必须用 注意，因为酵母样生物或细胞材料有时可以 模仿PC的外观。 使用抗体的特异性组织学染色 由于缺乏适当的技术， 抗体的 我们中的一个已经生产出了单克隆抗体制剂， 对抗人类电脑 这些抗体被用来开发一种特异性的 荧光抗体染色用于辅助诊断PC 肺炎 支气管灌洗标本正在由 传统甲苯胺蓝-O染色法和单克隆抗体间接法 免疫荧光方法。 我们还将开发一个 免疫过氧化物酶染色使用相同的实体制备。

Current methodology for the diagnosis of infection with Pneumocystis carinii (PC) relies on demonstration of organisms with typical morphology using special stains such as methenamine-silver, toluidine blue-O, etc. None of these stains are specific and they all must be interpreted with caution since yeast-like organisms or cellular materials can sometimes mimic the appearance of PC. Specific histologic staining using antibody directed against PC has not been possible due to the lack of appropriate antibody. One of us has produced monoclonal antibody preparations directed against human PC. These antibodies are being used to develop a specific fluorescent antibody stain to be used to aid in the diagnosis of PC pneumonia. Bronchial lavage specimens are being tested in parallel by traditional toluidine blue-O stain and by a monoclonal antibody indirect immunofluorescence procedure. We will also be developing an immunoperoxidase stain using the same entibody preparations.