INTERACTION OF GTP-BINDING MEMBRANE PROTEINS WITH CELLULAR COMPONENTS

ras癌基因的激活被认为是致病因子 在30%的人类肿瘤中。 然而，尽管有大量的工作， ras基因，包括至少7种不同ras的鉴定 基因和越来越多的ras样基因，几乎一无所知 ras蛋白在高等真核生物中的功能。 一个最近 一种被称为ARF的特征性蛋白质是腺苷酸环化酶的组分 系统，并与光栅共享几个功能，包括大小、位置和 能够与GTP结合。 的结合和水解的表征 采用ras和ARF进行鸟嘌呤核苷酸检测。 了系统性检索 然后进行，以确定增加 鸟嘌呤核苷酸的交换或水解 proteins. 这些研究应该定位这些蛋白质的细胞靶点 并可能确定这些蛋白质在细胞内的生理作用， 代谢或增殖。

Activation of the ras oncogene has been implicated as the causative agent in as many as 30% of all human tumors. Yet in spite of extensive work on the ras gene, including the identification of at least seven different ras genes and an increasing number of ras-like genes, almost nothing is known about the function of the ras proteins in higher eukaryotes. A recently characterized protein, termed ARF, is a component of the adenylate cyclase system and shares several features with ras, including size, location, and ability to bind GTP. Characterization of the binding and hydrolysis of guanine nucleoties by ras and ARF will be undertaken. A systematic search will then be conducted to identify factor(s) which increase either the exchange or hydrolysis of guanine nucleotides by these regulatory proteins. These studies should locate cellular targets for these proteins and may identify the physiological role of these proteins in cellular metabolism or proliferation.