CHARACTERIZATION OF AN ACID FAST BACTERIUM ISOLATED FROM ANIMAL DRINKING WATER

目的是表征和研究一种 从超纯水源中反复分离的抗酸（AF）细菌 为研究所的一些动物提供饮用水 生物体 被鉴定为龟分枝杆菌 生长特性 在肉汤培养基和固相培养基上进行比较。 Middlebrook 7 H9肉汤+ 白蛋白级分V和葡萄糖没有优于BHI或TSB， 得到了类似的结果。 营养肉汤是劣质的。 TGE琼脂为 有效或优于TSA、Mueller-Hinton和Middlebrook 7 H9肉汤， 以上+琼脂，是我们的标准琼脂。 对几种敏感性 确定了抗菌剂。 从这些结果来看，M. 在含有万古霉素（3 μ g/ml）+多粘菌素（10 μ g/ml）的TGE琼脂上 μ g/ml）。 初步结果表明，M.龟类不是 受这些浓度的影响。 媒体（TGE+VP）应证明是有用的 在检测水样和动物标本中是否存在M. 龟类。 无胸腺小鼠静脉接种M.龟鳖发育灶性 在接种后第2天（PID）至第12天，肝脏中可检测到肉芽肿 PID 50. 病变强度在第7天达到峰值。 AF微生物很少 在第11天后的切片中可见。 从肝脏中分离出AF细菌， PID 2、4、7（5/5）、11（35）、14（1/5）、21（2/5）、35（2/5）、50（0/5）。 将在自发性肝脏病变中搜索AF微生物。 这项工作是 由于观察到的起源不明的局灶性病变， 啮齿类动物的肝脏进行慢性生物测定根据NTP。 鉴定水传播细菌和分枝杆菌属，特别是作为 这一原因将允许在管理做法方面进行纠正。

The objective is to characterize and study the pathogenic potential of an acid fast (AF) bacterium repeatedly isolated from an ultrapure water source furnishing drinking water to some of the Institute's animals. The organism has been identified as Mycobacterium chelonae. Growth characteristics were compared in broth media and on solid phase media. Middlebrook 7H9 broth + albumin fraction V and dextrose gave no advantage over BHI or TSB which gave similar results. Nutrient broth was inferior. TGE agar was as effective or more so than TSA, Mueller-Hinton, and Middlebrook 7H9 broth as above + agar and is our standard agar. Sensitivity to several antimicrobials was determined. From these results the growth of M. chelonae on TGE agar containing vancomycin (3 Mug/ml) + polymyxin (10 Mug/ml) was examined. Preliminary results indicate that M. chelonae is not affected by these concentrations. The media (TGE+VP) should prove useful in examining water samples and animal specimens for the presence of M. chelonae. Athymic mice inoculated IV with M. chelonae developed focal granulomas detectable in the liver on post inoculation day (PID) 2 through PID 50. Intensity of lesions peaked by day 7. AF organisms were rarely seen in sections after day 11. AF bacteria were isolated from livers on PID 2, 4, 7 (5/5), 11 (3/5), 14 (1/5), 21 (2/5), 35 (2/5), 50 (0/5). Spontaneous liver lesions will be searched for AF organisms. This work is significant because of the focal lesions of undetermined origin observed in livers of rodents on chronic bioassays conducted under the NTP. Identifying water-borne bacteria and a Mycobacterium sp. in particular as the cause would allow for corrections in management practices.