MID-ATLANTIC REGIONAL IVEM AND IMAGE ANALYSIS RESOURCE

协调中压输电的区域资源 电子显微镜（300-400 kV，IVEM）和图像分析将 在费城成立，服务于大西洋中部地区， 国家 预期包埋的细胞和组织的切片高达 大约5微米厚，甚至更厚的 可以在这些电压下检查未嵌入的单元。 图像将 记录在胶片上或直接数字化用于图像分析。 的 图像分析的重点将放在三维分析上。 核心研究将是样本制备方法的开发 特别适用于通过电子显微镜研究细胞和组织， 这个电压范围，包括选择性染色剂的发展， 特殊的细胞成分。 图像分析核心研究将被定向 开发自动化数据采集和高效的用户交互 用于提取精确三维的图像处理方法 信息，包括形态测量信息，从多个图像， 结构，无论是连续显微照片或立体倾斜图像。 各种 提出了合作研究项目。 这些项目及其 赞助商将与核心研究和开发密切互动， 资源能力。 关注函和预期用途 已从纽约、新泽西、 宾夕法尼亚州、马里兰州和华盛顿，华盛顿特区的培训将在现场进行 当用户来到设施，并动手讲习班计划， 加强培训和传播关于 资源。 进一步传播成果和能力 资源将通过由和/或在 资源，并通过发表研究文章和评论文件。 总体目标是带来“最先进的”图像处理 与强大的工具并列， 各种类型和厚度的生物标本， 活跃和多样化的生物研究环境。

A Regional Resource for Coordinated Intermediate Voltage Transmission Electron Microscopy (300-400 kV, IVEM) and Image Analysis will be established in Philadelphia to serve the mid-Atlantic region of the country. It is expected that sections of embedded cells and tissues up to about 5 micrometers in thickness and even thicker whole mounts of unembedded cells can be examined at these voltages. Images will be recorded either on film or digitized directly for image analysis. The emphasis in the image analysis will be on analysis in three dimensions. Core research will be on development of specimen preparation methods especially suited for study of cells and tissues by electron microscopy in this voltage range, including the development of stains selective for particular cell components. Image analysis core research will be directed toward developing automated data acquisition and efficient user-interactive image manipulation methods for extracting accurate three dimensional information, including morphometric information, from multiple images of structures, either serial micrographs or stereo tilt images. A variety of collaborative research projects is proposed. These projects and their sponsors will interact closely with the core research and the development of Resource capabilities. Letters of interest and intended use of the Resource have been received from users in New York, New Jersey, Pennsylvania, Maryland, and Washington, D.C. Training will be done on-site when users come to the facility, and hands-on workshops are planned for additional training and dissemination of information on the capabilities of the Resource. Further dissemination of results as well as capabilities of the Resource will be done through symposia organized by and/or at the Resource and through publication of research articles and review papers. The overall goal is to bring "state of the art" image processing capabilities into juxtaposition with a powerful tool for examining biological specimens of a wide variety of types and thicknesses in an environment of active and diverse biological research.