PHAGOCYTIC CELL FUNCTION

利用中性粒细胞研究了吞噬细胞生物学的几个方面。 (PMN)和单核细胞。亚细胞定位的研究 FMET-Leu-Phe、C3bi以及细胞色素b提示这些成分 被包装在类似于特定颗粒的公共隔间中。在……里面 相关研究--一系列抗人血吸虫内脏的单抗 中性粒细胞质膜已经被开发出来，目的是识别 特定的分泌颗粒附着部位。渗出性中性粒细胞的研究有 发现的fmet-leu-Phe受体比血液PMN多五倍以上， 可能与从细胞内池中动员的受体有关。 在扩展我们早期关于中性粒细胞异质性的研究中， 研究了与中性粒细胞亚群结合的单抗31D8 慢性粒细胞白血病(CML)。数据表明有两个 慢性粒细胞白血病患者组；31D8亮或暗PMN组。它不是 明确慢性粒细胞白血病的异质性是否反映了克隆的差异 扩散。意外地出现了31D8抗原的缺失 预测慢性粒细胞白血病进展到加速期。 高免疫球蛋白E-复发性感染中IgE周转的研究 (约伯综合征)患者的IgE清除率降低 IgE升高。这对许多疾病有影响，在这些疾病中，IgE 抬高了。 在其他研究中，伽玛干扰素被证明是中性粒细胞的化学诱导剂。 和单核细胞，这可能与伽玛干扰素诱导 巨噬细胞巨细胞形成。

Several aspects of phagocyte biology have been studied using neutrophils (PMN) and monocytes. Investigation of the subcellular location of fmet-leu-phe, C3bi as well as for cytochrome b suggests these constituents are packaged in a common compartment similar to specific granules. In related studies a series of monoclonal antibodies against the inside of the neutrophil plasma membrane have been developed with the goal of identifying specific secretory granule attachment sites. Studies in exudate PMN have revealed greater than five-fold more fmet-leu-phe receptors than blood PMN, presumably related to receptors mobilized from the intracellular pool. In studies extending our earlier work on neutrophil heterogeneity a monoclonal antibody, 31D8, that binds to a subpopulation of PMN was studied in chronic myelogenous leukemia (CML). The data indicate there are two groups of CML patients; those with 31D8 bright or dull PMN. It is not clear whether or not the CML heterogeneity reflects differences in clonal proliferation. Unexpectedly absence of the 31D8 antigen appeared predictive of progression of CML to the accelerated phase. Studies of IgE turnover in the Hyperimmunoglobulin E-recurrent infection (Job's) syndrome have revealed decreased clearance of IgE in subjects with elevated IgE. This has implications for numerous diseases in which IgE is elevated. In other studies gamma interferon was shown to be a chemoattractant for PMN and monocytes and this may relate to gamma interferon's ability to induce macrophage giant cell formation.