MOLECULAR BIOLOGY OF HEPATOCELLULAR CARCINOMA

许多荷瘤动物都能产生针对相关独特抗原的抗体 致癌病毒导致肿瘤。这些抗原被称为 在乳头状病毒引起的肿瘤中发现了“新抗原”， 腺病毒和疱疹病毒。乙肝病毒，一种具有 疑似致癌潜力，不能传染给非灵长类动物，但 与乙肝病毒相关的肝癌患者可能会有抗体 一种乙肝病毒相关的新抗原，如果存在的话。使用一种肝癌细胞系 含有整合的HBVDNA，我们在血清中寻找免疫荧光抗体 肝癌患者的比例。大约7%的血清来自 乙肝表面抗原阳性的肝癌患者含有一种抗体，该抗体与一种 肝癌细胞系中的核抗原。这种抗原是在 另一个肝癌细胞系，也含有整合的乙肝病毒基因组，但 在另外两个缺乏乙肝病毒基因组的肝癌细胞系中则不存在。抗原性 (“乙肝病毒相关核抗原”：HBNA)正在进一步 其特征是确定它是否是转化基因的产物。一个 在一种人类肝癌细胞系中发现了不同的核抗原 不含HBVDNA。它被鉴定为与一名患有 乙肝表面抗原阴性的肝细胞癌。这种新的抗原已经 与HBNA相似的特征。

Many tumor-bearing animals develop antibodies to unique antigens associated with the oncogenic virus causing the tumor. These antigens, called "neoantigens," have been found in tumors caused by papovaviruses, adenoviruses, and herpes viruses. Hepatitis B virus, a hepadnavirus with suspected oncogenic potential, cannot be transmitted to non-primates but patients with HBV-associated hepatoma might be expected to have antibody to a HBV-associated neoantigen if one exists. Using a hepatoma cell line that contains integrated HBV DNA, we sought immunofluorescent antibody in sera of hepatoma patients. Approximately seven percent of sera from HBsAg-positive hepatoma patients contained an antibody that reacted with a nuclear antigen in the hepatoma cell line. This antigen was found in another hepatoma cell line that also contained integrated HBV genome but not in two other hepatoma cell lines lacking HBV genome. The antigen ("hepatitis B virus-associated nuclear antigen": HBNA) is being further characterized to determine if it is the product of a transforming gene. A different nuclear antigen was found in a human hepatoma cell line that did not contain HBV DNA. It was identified with serum from a patient with HBsAg negative hepatocellular carcinoma. The new antigen has characteristics similar to those of HBNA.