PROGRAM IN MACROMOLECULAR STRUCTURE, MOTION, CONTROL

该计划是针对高密度的大分子的结构研究。 蛋白质单晶形式的更大尺度分辨率数据 目前在核糖体和血浆等细胞器中可以评估的几何构型 膜。所采用的技术主要是绕射和解算。 X射线和中子的散射。除了平均时间之外 结构，这是这类工作的最直接输出，该程序将 特别是要研究聚合物各部分的运动 在较大的聚集体中，链和亚单位彼此相对。 运动可以部分地从变化的静态结构差异中推断出来 环境和结扎条件。特别令人感兴趣的是 催化循环中聚合酶类的特定侧链 以及核糖体在核糖体上的转位步骤 蛋白质合成过程中的信息。通过特定的方式对这些过程进行控制 将对配体进行调查。这些研究将结合实验和 对这些问题的理论探讨。

The program is directed at structural studies of macromolecules from high resolution data on proteins in the form of single crystals to the larger scale geometry presently assessable in organelles such as ribosomes and plasma membranes. The techniques to be employed center on diffraction and solution scattering of both X-rays and neutrons. In addition to the time-average structures, which are the most immediate output of such work, the program will be particularly aimed at studying the motion of various parts of the polymer chains and of subunits with respect to each other in the larger aggregates. Motion can, in part, be inferred from static structural differences under varied conditions of environment and ligation. Of special interest is the motion of specific side chains during catalytic cycles, of the polymerase class of enzymes along the nucleic acid chains, and the translocation step of ribosomes on the message during protein synthesis. The control of these processes by specific ligands will be investigated. The studies will combine both experimental and theoretical approaches to these problems.