ERROR-PRONE REPAIR IN BACTERIOPHAGE T4

这个项目的中心目标是分析容易出错的修复 在噬菌体T4中，与其他机制的相关探索， 诱变和一种新的机制，避免紫外线诱导的 杀人 T4中的大多数化学诱变和所有辐射诱变都是通过 并依赖于基因uvsW，uvsX和 uvsY，DNA复制所需的大部分或全部基因， 还有其他一些基因。 我们最近发现 X和Y基因的温度敏感等位基因， 差异地影响诱变和失活。 一些变种人X 蛋白质被分离出来，以确定它们的动力学 参数与诱变相关，与存活相关。 一些 已经或将要进行测试，以确定WXY的作用 系统中的光动力学诱导的诱变和杀伤，以确定是否 其它T4基因参与WXY作用（特别是基因46，47，49，58， 59和uvsZ），以更深入地探索uvsW基因的作用，并 描述了一个新发现的作用，在生存后，紫外线照射， T4基因32蛋白（主要的病毒DNA结合蛋白）。

The central objective of this project is the analysis of error-prone repair in bacteriophage T4, with related explorations into other mechanisms of mutagenesis and into a novel mechanism for avoiding ultraviolet-induced killing. Most chemical and all radiation mutagenesis in T4 occur via error-prone repair and depend on the functions of the genes uvsW, uvsX and uvsY, most or all of the genes required for DNA replication, and perhaps several other genes as well. We have recently characterized temperature-sensitive alleles of the X and Y genes, alleles that differentially affect mutagenesis and inactivation. Some of the mutant X proteins are being isolated in order to determine which of their kinetic parameters correlate with mutagenesis and which with survival. A number of tests have been or will be conducted to characterize the role of the WXY system in photodynamically induced mutagenesis and killing, to determine if other T4 genes are involved in WXY action (especially genes 46, 47, 49, 58, 59 and uvsZ), to more deeply explore the role of the uvsW gene, and to characterize a newly discovered role in survival after UV irradiation for the T4 gene 32 protein (the major viral DNA-binding protein).