MEASUREMENT OF TRACE-LEVEL METALS AND COMPLEXES IN BIOLOGICAL MILIEUX

分析方法已经被开发出来用于定量和 生物样品中金属元素的表征。分析 已对研究对象的样本中的钒进行了检测。 所获得的结果显示出很低的值，范围在 每克样品含钒纳克。这样的结果仅限于 因为样本量很少，所以非常困难 可用。对几种铂的络合行为特别感兴趣 临床上使用的不同化合物已经进行了研究。色谱学 技术被用来分离溶液中的物种。原子 使用电热原子化吸收光谱(ETA-AAS) 对总铂或单一分离物种进行定量。高效液相色谱分离 使用改进的极谱分析仪进行电化学检测 允许在水溶液中用 检出限约为50 ppb。已经开发出的分析方法 用于顺铂中铂物种行为的动力学研究 在溶液中。

Analytical methods have been developed for quantitation and characterization of metallic elements in biological samples. Analysis of vanadium in samples from human subjects under study has been conducted. Results obtained have shown very low values, in the range of nanograms-of-vanadium-per-gram of sample. Such results are obtained only with great difficulty because of the very samll amount of sample available. Specific interest in platinum complex behavior of several different compounds used clinically has been studied. Chromatographic techniques are used to make separations of species in solutions. Atomic Absorption spectroscopy with electrothermal atomization (ETA-AAS) is used to quantitate total platinum or single isolated species. HPLC separation with electrochemical detection using a modified polarographic analyzer permits quantitation of the parent CDDP complex in aqueous solutions with a detection limit of about 50 ppb. Analytical methods developed have been used to make kinetic studies of behavior of platinum species from cisplatin in solution.