ESTABLISHMENT OF HCV CONTAINING CELL CULTURE

丙型肝炎病毒（HCV）是世界上大多数慢性乙型肝炎的病原体， 输血后非甲、非乙病例，是一种正链RNA病毒。 黑猩猩的易感性使得连续采集血液 以及所有感染阶段的肝活检，但没有完整的病毒体 已经被令人信服地隔离了 一个可能的原因是， 证明这些代理（S）或检测病毒样抗原是非常 病毒在受感染宿主中复制的有限程度，如建议的 大多数血浆的感染性滴度较低。我们预计 肝细胞的原代培养物的发展可能被证明是敏感的。

The genome of hepatitis C virus (HCV), the etiologic agent of most post-transfusion non-A, non-B cases, is a positive stranded RNA virus. The susceptibility of chimpanzees has allowed collection of serial blood and liver biopsies from all stages of infection, yet no complete virions have been convincingly isolated. A likely reason for the failure to demonstrate these agent(s) or to detect virus-like antigens is the very limited degree of viral replication in the infected host, as suggested by low infectivity titer of most plasma. We anticipate that the development of a primary culture of liver cells might prove susceptible.