CONFOCAL MICROSCOPY OF CELLS

光学和电子学的最新进展达到了顶峰 激光共聚焦扫描显微镜(LCSM)的研制LCSM 为定位荧光探针提供无与伦比的分辨率 细胞。与先进但相对便宜的 在小巧的计算机上，制作三维图像相对容易 细胞内荧光标记结构的重建。我们有 我一直在使用最先进的BioRad MRC600 LCSM， 控制计算机。生成数据集的3-D重建 用LCSM获得，我们用VoxelView软件对一个硅图 Iris Indigo计算机。我们已将此系统用于各种不同的 应用，包括确定新的亚细胞分布 确定的蛋白质和新的细胞内转运 人工合成的膜蛋白。

Recent advances in optics and electronics have culminated in the development of the laser confocal scanning microscope (LCSM). The LCSM provides unparalleled resolution for localizing fluorescent probes in cells. In combination with advanced-yet relatively inexpensive and compact-computers, it is relatively easy to produce 3-dimensional reconstructions of fluorescently labeled structures in cells. We have been working with a state-of-the-art BioRad MRC 600 LCSM with its controlling computer. To produce 3-D reconstructions of data sets obtained with the LCSM, we use VoxelView software on a Silicon Graphics Iris Indigo computer. We have used this system for a wide variety of applications, including determining the subcellular distribution of newly defined proteins and following the intracellular trafficking of newly synthesized membrane proteins.