BIOMATERIAL EFFECTS ON PHAGOCYTE FUNCTION

PMN在体外处理人工表面时遇到人工表面 在输血前储存在血液采集设备和容器中， 在接受植入物和静脉导管的患者体内也是如此。 理想情况下，与血液成分接触的人造材料不应 显著改变细胞的功能反应或刺激 种植部位的炎症反应。这个项目的目标是 以确定亲水和疏水的衬底表面 生物材料上发生的修饰会改变人中性粒细胞(PMN) 能动性。 单项化学官能团在合成上的取代 表面对于检查特定化学物质的影响很有用。 关于细胞功能的部分。一个合作项目是与 J.J.希克曼(弗吉尼亚州麦克莱恩上汽集团的表面化学家) 对人工表面进行相对简单的化学修饰会改变PMN 迁徙行为。聚碳酸酯膜表面用 含多种官能团的自组装硅烷单分子膜 包括胺和疏水基团。聚碳酸酯表面 通过接触角测量和X射线光电子能谱进行评估 光谱学。对化学定义的表面进行了评估 对人中性粒细胞随机运动和趋化迁移的影响 N-甲酰基肽类趋化物质，FMLP。迁徙反应是 在四种聚碳酸酯表面上进行评估：1)对照，未经处理 聚碳酸酯，2)富羟基聚碳酸酯，3)亲水性烷基 硅烷(DETA)和4)疏水性烷基硅烷(13F)。 这些实验的结果表明，选定的亲水性 表面修饰，如DETA和羟基部分，增强了 人中性粒细胞的随机、未受刺激的运动，而疏水 表面修饰不会改变随机运动性。没有一个曲面 修饰可抑制PMN向FMLP的趋化迁移。这些 实验为定量研究提供了系统的基础 评价其他化学官能团对中性粒细胞的影响 迁移。未来的研究将包括对富含硫的化合物的评价 外源蛋白对中性粒细胞与中性粒细胞相互作用的影响 改性后的生物材料表面。

PMN encounter artificial surfaces ex vivo when they are processed and stored in blood collection devices and containers prior to transfusion, and in vivo in patients who receive implants and intravenous catheters. Ideally, artificial materials in contact with blood components should not significantly alter the functional responses of cells or stimulate an inflammatory response at the implant site. The goal of this project is to determine whether hydrophilic and hydrophobic substrate surface modifications that occur on biomaterials alter human neutrophil (PMN) motility. The substitution of individual chemical functionalities on synthetic surfaces is useful for examining the effects of specific chemical moieties on cell function. A collaborative project was undertaken with J.J. Hickman (a surface chemist at SAIC, McLean, VA) to determine whether relatively simple chemical modifications to artificial surfaces alter PMN migratory behavior. Polycarbonate membrane surfaces were modified with self-assembled silane monolayers containing various functionalities including amines and hydrophobic groups. The poly-carbonate surfaces were assessed by contact angle measurements and X-ray photoelectron spectroscopy. The chemically-defined surfaces were evaluated for their effects on human PMN random motility and chemotactic migration to the N-formyl peptide chemoattractant, FMLP. Migratory responses were evaluated on four polycarbonate surfaces: 1) Control, untreated polycarbonate, 2) hydroxyl-rich polycarbonate, 3) hydrophilic alkyl silane (DETA), and 4) hydrophobic alkyl silane (13F). The results of these experiments indicated that selected hydrophilic surface modifications such as DETA and hydroxyl moieties enhanced the random, unstimulated motility of human neutrophils, whereas, hydrophobic surface modifications did not alter random motility. None of the surface modifications inhibited PMN chemotactic migration to FMLP. These experiments provide the foundation of a system for quantitatively evaluating the effects of other chemical functionalities on PMN migration. Future studies will include the evaluation of sulfhydryl-rich surfaces and the effects of exogenous protein on PMN interactions with the modified biomaterial surfaces.