ANALYSIS AND SYNTHESIS OF CARBOHYDRATE ANTIGENS OF MYCOBACTERIUM TUBERCULOSIS

结核分枝杆菌是结核病的病原体， 每年造成大约300万例死亡， 多重耐药菌株感染的死亡率 该菌的产率超过80%。 M.结核病被许多 不寻常结构的多糖。 一种独特的成分， 多糖II由α，1，2-连接的D-葡萄糖残基组成。 设计了从多糖中分离该多糖的方案， 含有M.结核 我们证明了少校 可溶性多糖级分中的单糖组分， 分枝杆菌是阿拉伯糖、甘露糖和半乳糖。 多糖 还鉴定了含有甘露糖和葡萄糖的级分， 大致相等的金额。 我们证明了糖原的存在- 像多糖一样，作为一种胞外多糖， 分枝杆菌。 我们开发了一种化学合成 对应于推定的多糖II的寡糖。 我们 表明，对于这样的化合物，逐步合成的方法， 选择

Mycobacterium tuberculosis, the causative organism of tuberculosis is responsible for approximately three million death cases annually and the mortality rate of infections caused by multiple drug-resistant strains of this bacterium exceed 80%. M. tuberculosis is coated by a number of polysaccharides of unusual structure. A unique component termed polysaccharide II is composed of alpha, 1,2-linked D-glucose residues. Schemes were designed to isolate this polysaccharide from polysaccharide- containing extracts of M. tuberculosis. We demonstrated that the major monosaccharide components in soluble polysaccharide fractions from Mycobacteria are arabinose, mannose and galactose. A polysaccharide fraction was also identified which contains mannose and glucose in approximately equal amounts. We demonstrated the presence of a glycogen- like polysaccharide as one of the extracellular polysaccharides of Mycobacteria. We developed a method for the chemical synthesis of oligosaccharides corresponding to the putative polysaccharide II. We showed that for such compounds the stepwise synthesis is the method of choice.