ANATOMICAL ANALYSIS OF NEURONAL CIRCUITS

这个项目旨在提高我们对详细电路的理解 丘脑及其与觉醒、睡眠-觉醒周期的关系， 以及从皮质下中继器到大脑皮层的信息传递。 这个项目的具体目标是确定 潜在的神经调节性轴突分支与 丘脑网状核(TRN)内的神经元及其树突 在丘脑层内核(ILN)。目标是进一步 我们对丘脑门控神经回路的理解 功能。 利用凝集素标记小麦胚芽的顺行转运 辣根过氧化物酶(WGA-HRP)，丘脑轴突的分布 在中脑网状结构(MRF)中产生的 与桥脑网状区丘脑投射的比较 编队(PRF)。丘脑投射中的一些显著区别 在网状结构的这两个层面上都发现了发芽， 可能是前脑显著差异的基础的差异 脑干横断后已知的激活水平 在这两个层面上。 青光眼苍白球外投射的地形学研究 TRN是在过去一年内完成的。肩尾总长 GPE在TRN上的投影中的地形详细说明如下 逆行追踪法和顺行追踪法。 此外，从对侧投射的原点细胞 丘脑层内核(ILN)上的皮质被用 倒退运输的荧光染料。对侧的输入 发现ILN起源于额叶VI层深处的神经元 波瓣。

This project seeks to improve our understanding of the detailed circuitry of the thalamus and its relation to arousal, sleep-wakefulness cycles, and information transfer from subcortical relays to the cerebral cortex. The specific aim of this project is to identify the details of the relation of potential neuromodulatory axonal arborizations with the neurons and their dendrites in the thalamic reticular nucleus (TRN) and in the intralaminar nuclei (ILN) of the thalamus. The goal is to further our understanding of the neural circuitry underlying thalamic gating functions. Using anterograde transport of wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP), the thalamic distributions of axons arising in the mesencephalic reticular formation (MRF) were examined and compared with the thalamic projections arising in the pontine reticular formation (PRF). Some marked distinctions in the thalamic projections arising at these two levels of the reticular formation were found, distinctions which may underlie the striking differences in forebrain activation levels known to result following brainstem is transsections at these two levels. The study of topography in the external pallidal (GPe) projection upon the TRN was completed during this past year. The general rostrocaudal topography in the GPe projection upon the TRN was detailed with retrograde tracing methods as well as with anterograde tracing methods. In addition, the cells of origin of a projection from the contralateral cortex upon the thalamic intralaminar nuclei (ILN) was mapped using retrogradely transported fluorescent dyes. The contralateral inputs upon the ILN were found to arise from neurons deep in layer VI of the frontal lobe.