EFFECTS OF FOREIGN GENE EXPRESSION IN THE CNS ON AMYGDA-KINDLED RATS

该项目的目标是用腺病毒载体将基因送入大鼠的大脑。 联合病毒载体抑制杏仁核癫痫发作的实验研究 点燃火种。已经证明AAV是一种有效的基因递送系统 在中枢神经系统中，Bax mRNA的增加可能在细胞丢失中起重要作用 在癫痫发作后，进一步的实验将在 接下来的两步。首先，我们将把Bax反义基因重组到 腺相关病毒载体观察Bax反义寡核苷酸能否阻断细胞死亡或凋亡 培养的细胞。第二个主要步骤将是提供Bax反义 进入点燃的大鼠的海马区，看看它是否能阻止点燃的 癫痫发作。 我们可以使用AAV基因传递系统来转移任何感兴趣的基因 (即生长因子、CCK、Bc l-2等)进入老鼠的大脑。今年5月 对神经退行性疾病或精神疾病的基因治疗有潜在价值 障碍，并将进一步神经科学研究。如果AAV向量具有 在中枢神经系统中的高和长期表达，我们可以利用这个系统来 将我们感兴趣的基因(即CCK或生长因子)导入大鼠体内 大脑。这可能对肿瘤的基因治疗具有潜在的价值。 神经退行性或精神障碍，并将进一步神经科学 研究。

The goal of this project is to deliver genes into rat brain using adeno- associated virus (AAV) vector to quench seizures induced by amygdala kindling. Having shown that the AAV is an efficient gene delivery system in the CNS, and that increased Bax mRNA may be important in the cell loss of hippocampus after seizures, further experiments will be done in the next two steps. First, we will recombine the Bax antisense gene into the AAV vector to see if Bax antisense can block cell death or apoptosis in cultured cells. A second major step will be to deliver Bax antisense into the hippocampus of kindled rats to see if it can prevent the kindled seizures. We can use the AAV gene delivery system to transfer any gene of interest (i.e., growth factors, CCK, Bcl-2, etc.) into the rat brain. This may have potential value for gene therapy of neurodegenerative or mental disorders, and will further neuroscience research. If the AAV vector has high and long-term expression in the CNS, we can use this system to deliver our genes of interest (i.e., CCK or growth factor) into the rat brain. This may have potential value for gene therapy of neurodegenerative or mental disorders, and will further neuroscience research.