THREE-DIMENSIONAL HISTOLOGICAL RECONSTRUCTION

三维结构半自动采集系统 关于组织学材料的信息正在开发中。 的 系统应该比使用 连续切片，尽管分辨率可能有限。 简而言之， 包埋的组织块将相对于扫描电子 显微镜成像系统;块的表面将被成像， 存储的图像，连续切片将被内置的 切片机 因此，薄切片的处理和配准将 淘汰 人类和计算机模式识别将改变 将所得到的图像集转化为三维重建。 的 通过这种技术获得的Hermissenda crassicornis的图像 与相同组织的TEM图像很好地相关。

A semiautomatic system for acquiring three-dimensional structural information about histological material is being developed. The system should be faster and more reliable than techniques that use serial sections, although resolution may be limited. In brief, an embedded tissue block will be fixed relative to a scanning electron microscope imaging system; the surface of the block will be imaged and the image stored, and successive slices will be removed by a built-in microtome. Handling and registration of thin sections will thus be eliminated. Human and computer pattern recognition will transform the resulting set of images into a three-dimensional reconstruction. The images of Hermissenda crassicornis obtained by this technique correlate well with TEM images of the same tissue.