CORE--TISSUE ACQUISITION, PROCESSING AND ANALYSIS

组织采集、处理和分析核心是 病理科，其扩大的职责包括适当分配 和处理组织的研究超出了常规的诊断工作， 人类以及犬骨髓移植的解释 组织病理学 这个核心管理收集，记录，存储， 生物组织标本的处理和分析及其 识别数据和跟踪此赠款。 该单位协调所有 组织标本相关数据收集和分发给病理学， 其他弗雷德哈钦森癌症研究中心（FHCRC）研究实验室。 例如，骨髓被分成血液学标本， 病理学、细胞遗传学、细胞相互作用、流式细胞术、细胞因子 实验室和其他实验室。 这些研究中大约有一半是 不可收费，部分可收费研究无法收回。 长期随访的标本是由独特的经验， 病理工作人员是否可以收取账单。 处理包括 冷冻、固定、包埋、储存和适当分配， FHCRC内部实验室的子集。 内部可用的分析方法 病理学包括研究免疫组织化学，原位杂交（如 对于Y染色体DNA），聚合酶链反应（PCR），荧光， 原位杂交（FISH）和特化移植物抗宿主病（GVHD） 研究（例如） TUNEL法检测人和犬骨髓移植物中细胞凋亡 受惠人士 自动化领域新方法的研究与开发 免疫组织化学、原位杂交和PCR提供了“尖端” 技术，以支持研究项目的研究， 程序项目。

The Tissue Acquisition, Processing and Analysis Core is an outgrowth of the pathology section whose expanded responsibilities include proper allocation and processing of tissue for research beyond the routine diagnostic work in humans as well as interpretation of canine bone marrow transplant histopathology. This core manages the collection, logging, storage, processing and analysis of biological tissue specimens and their identifying data and tracking for this grant. The unit coordinates all tissue specimen related data collection and distribution to pathology and other Fred Hutchinson Cancer Research Center (FHCRC) research laboratories. Bone marrow, for example, is divided into specimens for hematology, pathology, cytogenetics, cellular interactions, flow cytometry, cytokine laboratories, and other laboratories. About half of these studies are nonbillable and in a fraction the billable studies are uncollectable. Long-term follow-up specimens are seen by the uniquely experienced pathology staff whether billing is collectable or not. Processing includes freezing, fixation, embedding, storage, and appropriate distribution to subset of intramural FHCRC laboratories. Analytic methods available within pathology include research immunohistochemistry, in situ hybridization (as for Y chromosome DNA), polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH), and specialized graft-versus-host disease (GVHD) studies (e.g.) Tunel stain for apoptosis) in human and canine marrow graft recipients. Research and development of new methodology in automated immunohistochemistry, in situ hybridization, and PCR provide "cutting edge" technology to support the studies of the research projects within the program project.