CORE--VECTOR

核心A(向量)将在设计、施工和 基于腺病毒的基因治疗载体的结构验证 (Ad)。核心的主要作用是提供服务、知识和 给调查人员提供生物材料，这样他们就可以专注于 他们项目的生物学方面，而不是广告的技术 向量构造。出于这个原因，核心将满足以下要求 计划中所有四个项目的角色：大规模执行 参与项目的载体准备工作(具体目标50 准备工作/年)根据核心使用量分配和 可用的资源；通过导入创建新的基因治疗载体 技术和/或新的转基因和衣壳的改进 对参与项目(目标)很重要的修改 18/年)；提供所有阶段的标准操作程序 所有广告载体的构建；项目科学家的教育 通过与资深核心科学家的教程构建载体 和跟踪核心技术人员；提供经过验证的生物样本 构建载体的样本包括质粒、细胞系和 开始向量，并维护一个描述这些材料的数据库。

Core A (Vector) will provide assistance in the design, construction and structural verification of gene therapy vectors based on adenovirus (Ad). The major role of the core is to provide service, knowledge and biological materials to the investigators so they may focus on the biological aspects of their projects rather than the technology of Ad vector construction. For this reason the core will fulfill the following roles for all four of the projects in the program: Perform large scale vector preparations for the participating projects (target 50 preparations/year) in accordance with the core usage allocations and resources available; Create new gene therapy vectors by importing improvements in the technology and/or new transgenes and capsid modifications that are important for the participating projects (target 18/year); Provide Standard Operating Procedures for all phases in the construction of all Ad vectors; Education of project scientists in construction of vectors through tutorials with senior core scientists and shadowing core technicians; Supply verified samples of biological samples for the vector construction including plasmids, cell lines and starting vectors, and maintain a database describing those materials.