HORMONAL CONTROL OF LUNG DEVELOPMENT--PTHRP BIOLOGY

描述：（改编自申请人摘要）拟定研究 是专门设计来测试以下假设：（1） 胎儿II型细胞中牵张启动的转导触发PTHrP （2）PTHrP刺激胎肺成纤维细胞成熟， 受体依赖性机制;（3）PTHrP刺激产生 促进II型细胞增殖的间充质细胞因子和生长因子 成熟 脂蛋白脂酶、糖皮质激素受体和 PTHrP缺陷型小鼠PTHrP调节的成纤维细胞生长因子和细胞因子 肺组织和成纤维细胞将在mRNA和蛋白质水平上进行研究 以确定该模型中哪些功能受到影响。

DESCRIPTION: (Adapted from the applicant's abstract) The proposed studies are specifically designed to test the following hypotheses: (1) stretch-initiated transduction in fetal type II cells triggers PTHrP production; (2) PTHrP stimulates fetal lung fibroblast maturation by receptor-dependent mechanisms; (3) PTHrP stimulates production of mesenchymal cytokines and growth factors which promote type II cell maturation. Elaboration of lipoprotein lipase, glucocorticoid receptor, and PTHrP-regulated fibroblast growth factors and cytokines by PTHrP-deficient lung tissue and fibroblasts will be studied at the mRNA and protein levels to determine which functions are affected in this model.