DEFENSE AGAINST NEONATAL INFECTION VIA MATERNAL IMMUNITY

研究了母亲免疫接种对婴儿的影响， 幼龄小鼠对9V型肺炎球菌后续免疫应答 多糖（PS）、肺炎球菌溶血素（ply）和9V PS-ply缀合物。 年轻 来自用9V型PS、灭活肺炎球菌溶血素或 9V PS-层片结合物在妊娠期间接受额外剂量的 断奶时抗原相同。 这些小鼠产生较高特异性IgM抗体 而不是来自未免疫母亲的幼鼠。 此外，来自 在怀孕期间用19 F型PS-层结合物免疫的母亲， 在出生后接受额外剂量的相同缀合物， 19 F PS IgG和IgM抗体高于未免疫的幼龄小鼠 妈妈们 5 x 104挑战后快速细菌清除 cfu/剂量的19 F肺炎球菌在接受了 与未免疫的对照相比，围产期期间的19 F PS缀合物 对照 这些结果表明， 肺炎球菌PS-蛋白缀合物在妊娠期间和施用 对出生后的幼鼠额外的缀合物赋予保护性， 小鼠抗肺炎球菌感染的免疫力。

Studies were conducted on the effect of maternal immunization on the subsequent immune response of young mice to pneumococcal type 9V polysaccharide (PS), pneumolysin (ply), and 9V PS-ply conjugate. Young mice from mothers immunized with type 9V PS, inactivated pneumolysin, or 9V PS-ply conjugate during pregnancy received an additional dose of the same antigen at weaning. These mice produced higher specific IgM antibody than young mice from unimmunized mothers. Furthermore, young mice from mothers immunized with type 19F PS-ply conjugate during pregnancy that received an additional dose of the same conjugate after birth produced higher 19F PS IgG and IgM antibodies than young mice from unimmunized mothers. Rapid bacterial clearance following challenge with 5 x 104 cfu/dose of 19F pneumococci was observed in young mice that had received 19F PS conjugate during perinatal period as compared to the non-immunized controls. These results indicate that maternal immunization with pneumococcal PS-protein conjugate during pregnancy and administration of an additional conjugate to young mice after birth conferred protective immunity against pneumococcal infection in mice.