CELLULAR AND MOLECULAR STUDIES OF RENAL TRANSPORT

寻求对上皮性和综合性的广泛和综合方案的支持 肾脏研究。将使用广泛的方法进行调查 电解质和液体通过极化细胞的传输机制， 尤其是肾单位的。组件项目将调查 分子运输操作(离子通道、泵和耦合 运输)利用孤立的小管调节钠、钾、氯和氢的运输， 肾和肾小球系膜细胞培养，单管细胞，肾膜 和运输蛋白。广泛的研究范围不仅包括 努力确定运输蛋白的分子结构，但也 了解支配它们分布和插入的机制 进入上皮膜。离子转运体和离子转运体之间的相互作用 他们的规定也将被研究。 几个核心设施将支持强交互研究 项目。这些核心单元包括一个用于开发和维护的单元 电子设备、用于成像技术的扩展单元和电池 文化核心设施。 通过提供关于分子和细胞的新信息 离子和流体的传输机制，我们的研究也将改善 对肾脏疾病和临床电解质的认识和治疗 精神错乱。它们还可以为开发新的 利尿剂的有害副作用较少。

Support is sought for a broad and integrated program in epithelial and renal research. A wide range of approaches will be used to investigate the mechanisms of electrolyte and fluid transport across polarized cells, especially those of the nephron. The component projects will investigate the molecular transport operations (ion channels, pumps and coupled transport) mediating Na,K, C1 and H transport, using isolated tubules, renal and mesangial cell cultures, single tubule cells, renal membranes and transport proteins. The broad range of studies will include not only effort to identify the molecular structure of transport proteins but also to understand the mechanisms governing their distribution and insertion into epithelial membrane. The interaction between ion transporters and their regulations will also be studies. Several core facilities will support the strongly interactive research projects. These core units include one for developing and maintaining electronic equipment, and expanded unit for imaging techniques and a cell culture core facility. By providing novel information about the molecular and cellular mechanisms of ion and fluid transport, our studies also will improve the understanding and treatment of renal diseases and clinical electrolyte disorders. They also may provide a rational basis for developing new diuretics with fewer harmful side effects.