ULTRAVIOLET RADIATION INDUCED SKIN CANCER

工作综述：UV-B辐射可能会抑制 迟发型超敏反应的诱导和诱发 (DTH)。我们已经开发出一种使用激光的定量方法。 UV-AB诱导的多普勒血流灌注成像分析 利用前文中的激发响应抑制潜伏期 结核菌素使个体致敏以调查剂量 紫外线剂量与辐射诱导的反应关系 抑制或抵抗紫外线诱导的抑制。十九 健康志愿者在所描绘的四天内每天接受照射 上背部有紫外线-AB辐射的区域。UV-AB剂量水平 被调整为提供60或120 mJ/cm2的UV-B辐射。论 第四天，每个试验点一个辐射试验点和一个非辐射试验点 在每个人身上随机选择紫外线剂量并注射 皮内注射结核菌素。分析了测试反应，并 在96h临床和DPI进行量化。结果表明，该系统具有较高的可靠性。 UV-AB辐射对结核菌素诱导期的抑制作用 DTH存在剂量依赖性和个体差异 在免疫抑制作用下的紫外线辐射。低剂量 UV-B，观察到了敏感和抗性两种表型 1：1。然而，加倍的UV-B增加了 在一些个体中的压抑，在其他人中诱导压抑 具有抵抗力，或诱导他人产生抵抗力或耐受性 对较低剂量的紫外线敏感。我们假设 紫外线诱导抑制迟发型超敏反应的阈值及其诱导 可能阻断诱导的T抑制细胞的数量有很大的差异。 因此，UVR和UVR剂量的表征 感病或抗病诱导的反应关系 必须确定才能充分表现出这些 特征。

Summary of Work: UV-B radiation may suppresses the induction and elicitation of delayed-type hypersensitivity (DTH). We have developed a quantitative method using laser Doppler perfusion imaging analysis (DPI)of UV-AB induced suppression of DTH using the elicitation response in previously tuberculin sensitized individuals in order to investigate the dose response relationship between UV dose and induction of suppression or resistance to UV induced suppression. Nineteen healthy volunteers were irradiated daily for four days on delineated areas on the upper back with UV-AB radiation. UV-AB dose levels were adjusted to deliver 60 or 120 mJ/cm2 UV-B radiation. On the fourth day, one irradiated and one unirradiated test site for each UV-dose were chosen at random on each individual and injected intradermally with tuberculin. Test reactions were analyzed and quantified at 96 h clinically and with DPI. The results indicate that UV-AB radiation can suppress the elicitation phase of tuberculin DTH and that there are dose dependent and individual differences in the immune suppressive effect of the UV-radiation. At low dose UV-B, both the susceptible and resistant phenotypes were observed at an ratio of 1:1. However, doubling UV-B increased the suppression in some individuals, induced suppression in others that were resistant, or induced a resistance or tolerance in others that were susceptible at the lower dose of UV. We hypothesize that the thresholds for UV induced suppression of DTH and the induction of T suppressor cells that may block elicitation vary considerably. Thus, characterization of both the UVR and the UVR dose response relationship for induction of susceptibility or resistance must be determined in order to adequately phenotype for these traits.