MOLECULAR EVALUATION OF COBALT SULFATE LUNG NEOPLASMS

工作总结：硫酸钴诱导了较高的 男性肺泡/细支气管腺瘤和癌发病率 雌性小鼠暴露于不同浓度的硫酸钴 与对照相比。肺肿瘤的DNA样本， 对K-ras基因的点突变进行测序唯一点 在K-ras外显子2中检测到突变。今后在这方面的工作 项目包括确定独特的机制， 突变和表征潜在肿瘤中的遗传改变 可能与人类接触有关的抑制基因。的 这些研究的第一部分的结果被印在NTP上 1996年12月审查委员会。曼尼托巴正在进行中。

Summary of Work: Cobalt sulfate induced a higher incidence of alveolar/bronchiolar adenomas and carcinomas in male and female mice exposed to various concentrations of cobalt sulfate compared to controls. DNA samples from lung neoplasms were sequenced for point mutations in the K-ras gene. Unique point mutations were detected in K-ras exon 2. Future work on this project includes determining the mechanism for the unique mutations and characterizing genetic alterations in potential tumor suppressor genes which may be relevant to human exposure. The findings for the first part of these studies were printed in the NTP Review Board in December 1996. Manuscript is in process.