SPECTROSCOPY CONSOLE AND GRADIENTS FOR 7T NMR SYSTEM

我们正在申请资金购买Bruker Avarice DBX NMR 光谱/成像控制台和具有RF线圈屏蔽梯度装置， 放置在位于Yale Magnetic中的7.OT 20 cm孔磁体上 共鸣中心。 7.OT系统一直走在前列， MRS和MRI方法的发展和应用，以了解 代谢的调节以及代谢在病理学中如何改变。 项目部分所述的研究旨在解决 基础生物化学/生理学和临床的前沿问题 科学包括大脑功能活动的能量需求， 抗癫痫化合物与GABA代谢的相互作用， 功能，新生儿皮质对缺氧的代谢适应， 改变肌肉和肝脏碳水化合物代谢在 糖尿病的发病机制，以及代谢在调节中的作用 神经递质谷氨酸和伽马氨基丁酸的混合体 深入了解 从这些研究中获得的代谢调节指导我们 类似方法在人类病理学研究中的应用 我们的2.1T全身系统目前配备了 一台Avance控制台 目前，我们有能力进一步提高MR 过时的控制台和梯度设置阻碍了该方法。的 要求的设备将使我们能够继续开发和应用国家 的艺术NMR方法的研究问题中所描述的， 提议 在许多实验中，新设备将导致2-4倍 灵敏度提高和空间分辨率提高。 的 要求的设备提供的MRS和MRI的改进将允许 耶鲁大学的研究人员将保持在基础和临床研究的最前沿 新陈代谢和生理学的基础。

We are requesting funds to purchase a Bruker Avarice DBX NMR spectroscopy/imaging console and shielded gradient set with rf coil to be placed on a 7.OT 20 cm bore magnet located in the Yale Magnetic Resonance Center. The 7.OT system has been in the forefront in the development and application of MRS and MRI methods to understand the regulation of metabolism and how metabolism is altered in pathology. The studies which are described in the project section are addressing cutting edge issues in basic biochemistry/physiology and clinical science including the energy requirements of brain functional activity, the interaction between anti epileptic compounds and GABA metabolism and function, metabolic adaptations of the neonatal cortex to hypoxia, the role of altered muscle and liver carbohydrate metabolism in the pathogenesis of diabetes, and the role of metabolism in the regulation of neurotransmitter glutamate and GABA pools. The insights into metabolic regulation obtained from these studies has guided us in the application of similar methods in the study of pathology in human patients on our 2.1T whole body system which is presently equipped with an Avance console. Presently our ability to further improve the MR methodology is hampered by an obsolete console and gradient set. The requested equipment will allow us to continue to develop and apply state of the art NMR methodology to the research problems described in this proposal. In many experiments the new equipment will lead to a 2-4 fold improvement in sensitivity and spatial resolution improvement. The improvements in MRS and MRI provided by requested equipment will allow Yale researchers to stay in the forefront of basic and clinical studies of metabolism and physiology.