MECHANISM OF UREASE METALLOCENTER BIOSYNTHESIS

我们试图描述尿素酶脱辅基蛋白之间的相互作用 (Apo)以及四种尿素酶所需的辅助蛋白 活化、UreD、UreE、UreF和UreG。 我们确定 三种辅助蛋白与Apo形成以下复合物： UreD-Apo、UreF-UreD-Apo和UreG-UreF-UreD-Apo，并计划分析 肽-肽相互作用的位点 包括交联肽的质谱分析。 在 此外，我们计划进行化学足迹研究， 在存在下检查Apo上的化学修饰位点， 各种复杂的推断，哪些网站是掩盖了 辅助蛋白

We seek to characterize the interactions between urease apoprotein (Apo) and four accessory proteins that are required for urease activation, UreD, UreE, UreF, and UreG. We have established that three of the auxiliary proteins form the following complexes with Apo: UreD-Apo, UreF-UreD-Apo, and UreG-UreF-UreD-Apo, and plan to analyze the sites of peptide-peptide interaction by a combination of methods including mass spectrometric analysis of crosslinked peptides. In addition, we plan to carry out chemical footprinting studies in which sites of chemical modification on Apo are examined in the presence of the various complexes to deduce which sites are obscured by the accessory proteins.