ENHANCED ELECTORHEOLOGICAL FLUIDS USING HYERPTHERMOPHILIC BIOTECHNOLOGY

微生物腐败希瓦氏杆菌可以与多种细菌一起生长 末端受体，包括氧、硝酸盐、亚硝酸盐、TMAO、Fe(3+)、 这种多样性允许末端的合成 还原酶可被外加受体诱导。学习电子 腐烂链球菌的运输链，我们使用的是低温EPR 不同频率的光谱分析。事实证明， 根据所研究的可及电子受体， 微生物产生了不同的电子载体。通过以下方式实现增长 Fe(3)作为末端电子受体诱导了 多血红素细胞色素2型低分子(约10kD)和高分子(70kD) 重量。这两种细胞色素的每个蛋白质分子都有四个血红素基团。 我们用低温电子顺磁共振波谱研究了 同一蛋白质分子中的血红素基团。

Microorganism Shewanella putrefaciens can grow with a variety of terminal acceptors, including oxygen, nitrate, nitrite, TMAO, Fe(3+), Mn(4+), etc. Such diversity admits that synthesis of terminal reductases could be induced by applied acceptors. To study electron transport chain of S. putrefaciens, we used low temperature EPR spectroscopy at different frequencies. It has been shown that, depending on the accessible electron acceptor studied, the microorganism developed different electron carriers. The growth with Fe(3) as the terminal electron acceptor induced the appearance of multiheme cytochromes type 2 low (ca 10 kD) and high (70 kD) molecular weight. Both cytochromes have four heme groups per protein molecule. We use low temperature EPR spectroscopy to study interactions between heme groups in the same protein molecule.