MASS SPECTROMETRIC ANALYSIS OF PROBLEM AMINO ACID SEQUENCES

许多蛋白质氨基酸序列无法进行序列分析 常规埃德曼测序程序。困难出现了，因为 例如，通过阻止或其他修改并通过 重复的氨基酸残基。我们用的是质谱仪 碰撞诱导解离串联质量法 光谱分析从这种难处理的蛋白质中获得序列。一个 描述这部作品的手稿已经出版，J.Godinho da Silva， 首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容 一种新的过敏性2S白蛋白的氨基酸序列 30 kDa大小的蓖麻贮藏蛋白 《生物化学与生物物理档案》336(1996)10-18。

Many protein amino acid sequences defy sequence analysis by the convention Edman Sequencing procedure. The difficulties arise, for example, through blocking or other modifications and through repetitive amino acid residues. We are using mass spectrometric methods such as collision-induced dissociation tandem mass spectrometry to obtain sequences from such intractable proteins. A manuscript describing this work has been published J.Godinho da Silva, Jr., O.L.T. Machado, C. Izumi, J.C. Padovan, B.T. Chait, U.A. Mirza & L.J. Greene "Amino acid sequence of a new allergenic 2S albumin storage protein from Ricinus communis which is part of a 30 kDa precursor protein" Archives of Biochem. & Biophys. 336 (1996) 10-18.