SIGNAL TRANSDUCTION & TRANSCRIPTION PROTEIN (STAT)

重组STAT1 C-端片段的鉴定 MALDI-MS分析蛋白质烷基化程度的测定 以及蛋白质的磷酸化。本项目的续展 将包括测定下列氨基酸残基 利用光交联和MALDI-MS多肽与DNA结合的重要意义 映射。描述这项工作的一篇论文发表在美国。 文克梅尔，S.L.科恩。首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容 体外激活的STAT1(、STAT1(和))的DNA结合 NH2-末端结构域之间截断的STAT1相互作用稳定 两个二聚体与串联DNA位点的结合>《EMBO J.15(1996)5616- 5626号。

Characterization of a C-terminal fragment of the recombinant STAT1 protein by MALDI-MS. Determination of the extent of alkylation as well as phosphorylation of the protein. Continuation of this project will include the determination of amino acid residues that are important for DNA binding using photocrosslinking and MALDI-MS peptide mapping. A paper describing this work has been published U. Vinkemeier, S.L. Cohen. I. Moarefi, B.T. Chait, J. Kuriyan & J.E. Darnell "DNA binding of in vitro activated Stat1(, Stat1( and truncated Stat1 interaction between NH2-terminal domains stabilizes binding of two dimers to tandem DNA sites." EMBO J. 15 (1996) 5616 - 5626.