MILLISECOND TIME-SCALE HEAT-INDUCED DENATURATION OF PROTEINS

我们以前使用ESI-MS来探测 蛋白质在平衡溶液中的构象状态。 的 这一探测的基础是观察到的电荷状态， 气相中的电喷雾蛋白质取决于构象 蛋白质在溶液中的状态 加热电喷雾中的蛋白质 水滴。 这项研究表明，特殊条件是 所需的诱导蛋白质的构象变化， 毫秒时标 相反，因为构象的变化， 蛋白质通常发生的时间尺度是缓慢的相比， 电喷雾液滴的寿命，它证明是可行的推断 关于构象的信息。 蛋白质的变化 一份描述我们公司的手稿， 发表了结果（Miraz和Chait，Int J Mass Spectrometry and Ion Processing 162，（1997）173-181）

We have previously used ESI-MS to probe changes in the conformational states of proteins in well-equilibrated solutions. The basis for this probe was the observation that the charge states of electrosprayed proteins in the gas phase depend on the conformational states of the protein in solution. proteins in heated electrospray droplets. This study demonstrates that special conditions are required to induce conformational changes in proteins on the millisecond time scale. Conversely, because conformational changes in proteins often occur on time-scales that are slow compared with the life-time of electrospray droplets, it proves feasible to deduce information concerning conformational. changes of proteins in the electrospray solution prior to spraying A manuscript describing our results was published (Miraz and Chait, Int J Mass Spectrometry and Ion Processing 162, (1997) 173-181)