MOTOR PROTEINS IN ASTRAL & SPINDLE FORCES IN FUNGUS NECTRIA HAEMATOCOCCA

(部分由国家科学基金会MCB 9408249至J.Aist提供支持)。为了过去 几年来，艾斯特博士和他的博士生井上聪一直使用 BMIRR的激光显微外科/光学捕捉组件 在他们的研究计划中广泛存在。今年的目标是 确定分离主轴的力产生的位置 在真菌红球线虫B后期的极点，并到 探讨胞质动力蛋白在核有丝分裂中的作用 移民和核定位。要实现前两个目标 晚期采用激光显微手术切断纺锤体 在对照细胞和细胞质动力蛋白突变体中。要实现 后两个目标是用光学陷阱来测量相对 阻止核迁移和转移核所需的力量， 控制者和变种人。今年有两篇论文发表在 这些研究的结果，只能在BMIRR进行 由于其独特的基于DIC的激光显微手术系统。井上先生， S.，B.G.Turjo，O.C.Yoder和J.R.Aist(1998)。真菌的作用 菌丝生长、微管组织、纺锤体极体中的动力蛋白 机动性和核迁移。《细胞科学》111：1555-1566。 书名/作者Inoue，S.，O.C.Yoder，B.G.Turjo和J.R.Aist。1998年。一个 体内有丝分裂酯形成所需的细胞质动力蛋白。J. 细胞科学，111：2607-2614。

(Supported in part by NSF MCB 9408249 to J. Aist). For the past several years Dr. Aist and his Ph.D student, Satoshi Inoue, have used the laser microsurgery/optical trapping component of the BMIRR extensively in their research program. This year's goals were to determine where the forces are generated that separate the spindle poles during anaphase B in the fungus Nectria haematococca, and to investigate the role of cytoplasmic dynein in mitosis, nuclear migration and nuclear positioning. To achieve the first two goals laser microsurgery was used to sever spindles during anaphase in control cells and in cytoplasmic-dynein mutants. To achieve the latter two goals the optical trap was used to measure the relative forces needed to arrest nuclear migration, and displace nuclei, in controls and mutants. Two papers were published this year on the results of these studies, which could only be conducted at the BMIRR because of its unique DIC-based laser microsurgery sy stem. Inoue, S., B.G. Turgeon, O. C. Yoder and J. R. Aist (1998). Role of fungal dynein in hyphal growth, microtubule organization, spindle pole body motility and nuclear migration. J. Cell Science 111:1555-1566. Inoue, S., O.C. Yoder, B.G. Turgeon and J. R. Aist. 1998. A cytoplasmic dynein required for mitotic aster formation in vivo. J. Cell Science., 111:2607-2614.