NEUROTROPHIC FACTORS & RECEPTORS IN DEFINED NEURAL NETWORKS

这一合作提案涉及到结构性的决定， 神经营养因子与其受体的结合位点， 化学交联，配体受体复合物的分离， 他们的蛋白水解消化和结构分析先进的 质谱分析方法。 此外，质谱方法 将被开发来确定合成神经营养素的纯度 以及监测分泌、摄取和逆行转运 特定的神经营养因子的作用。 的碳-14标记 神经营养因子将被用来鉴定受体亚型， 凝胶和加速器质谱法，然后是受体 氨基酸串联质谱鉴定 标记条带的测序。

This collaborative proposal involves the structural determination of the binding sites of neurotrophins to their receptors using chemical cross-linking, isolation of the ligand receptor complexes, their proteolytic digestion and structural analysis by advanced methods of mass spectrometry. In addition, mass spectrometric methods will be developed to establish the purity of synthetic neurotrophins as well as to monitor the secretion, uptake and retrograde transport of specific neurotrophins in neurons. Carbon-14 labelled neurotrophins will be used to identify receptor sub-types using 2-d gels and accelerator mass spectrometry followed by receptor identification using tandem mass spectrometry for amino acid sequencing of labelled bands.