ENERGETICS OF SMALL RNA PSEUDOKNOT

伪结是主要的结构主题之一，它允许 将RNA折叠成相当紧凑的形式。伪结点也是 调控某些mRNAs的重要信号。在……里面 莫洛尼鼠白血病病毒，一个假结已知是重要的 在调节信使核糖核酸的通读程度上，因此建立了 GAG和Gag-Poll蛋白的生成量。其作用机制是 READTHROW事件未知。一般来说，只有几个 关于伪结折叠的研究不完全，本研究的目的是 对折叠的热力学描述比较完整 假结的能量学。量热数据是必不可少的 这是对已有的紫外线高色度数据的补充。这个 这两个数据集的组合将有助于确定 这种RNA的展开。它是 设想这样的数据将在确定机制时有用 通读。

Pseudoknots are one of the major structural motifs which allow RNAs to fold into rather compact forms. Pseudoknots are also important signals governing the regulation of certain mRNAs. In Moloney Murine Leukemia virus, a pseudoknot is known to be important in regulating the degree of mRNA readthrough, hence establishing the amount of gag and gag-pol protein being made. The mechanism of the readthrough event is unknown. In general, there are only a few incomplete studies on the folding of pseudoknots, and this study aims to be a relatively complete thermodynamic description of the folding energetics of a pseudoknot. The calorimetry data is an essential complement to the UV hyperchromcity data already in hand. The combination of these two data sets will aid in determining the unfolding of this RNA. It is envisioned that such data will be useful in determining the mechansim of readthrough.