HOX10 GENES REGULATE LUMBAR SPINAL CORD PATTERNING

描述(改编自申请者的描述)：所描述的实验 在这份提案中，我们设计了一套三个并列的角色 HOX基因在形成发育中的腰椎脊髓中的作用。这些基因， Hoxa10、Hoxc10和Hoxd10在广泛的结构域中表达，包括 并选择腰椎进行分析，因为它们是一个完整的 并列的群和一起很可能提供所有的图案 由霍克斯复合体内的这个空间位置决定的信息。三 将解决具体目标：1)确定区域和地方模式 Hoxa10、Hoxc10和Hoxd10在腰椎发育过程中的表达 脊髓，2)鉴定腰髓中的细胞群， 表达Hoxca10、Hoxc10或Hoxd10，以及3)证明失活 Hoxa10、Hoxc10或Hoxd10改变特定细胞的产生和放置 腰椎脊髓中的种群。这些实验将测试 假设调控基因的表达和活性 转录，如HOX基因，建立了区域和细胞的身份 发育中的中枢神经系统。

DESCRIPTION (Adapted from applicant's description): The experiments described in this proposal are designed to examine the role of a set of three paralogous Hox genes in patterning the developing lumbar spinal cord. These genes, Hoxa10, Hoxc10, and Hoxd10 are expressed in broad domains encompassing the lumbar spinal cord and were chosen for analysis because they are a complete paralogous group and together are likely to provide all of the patterning information dictated by this spatial location within the Hox complex. Three specific aims will be addressed: 1) to define the regional and local patterns of expression of Hoxa10, Hoxc10, and Hoxd10 in the developing lumbar spinal cord, 2) to identify the populations of cells in the lumbar spinal cord which express Hoxca10, Hoxc10, or Hoxd10, and 3) to demonstrate that inactivation of Hoxa10, Hoxc10, or Hoxd10 alters the production and placement of specific cell populations in the lumbar spinal cord. These experiments will test the hypothesis that the expression and activity of genes which regulate transcription, such as Hox genes, establish the regional and cellular identity of the developing central nervous system.