GENETICS OF BACTERIAL THIOL REDOX PROTEINS

描述：（改编自研究者摘要） 本文介绍了大肠杆菌巯基氧化还原蛋白的研究。杆菌 作为探索过程特征的方法 蛋白质穿过细胞质膜的过程。具体 目标是：1.利用突变分析来研究蛋白质 在正常细胞质的易位过程中折叠 硫氧还蛋白进入E. coli周质; 2.使用突变分析， 生物化学研究，以确定结构之间的关系 以及两种细胞包膜蛋白DsbA和DsbB的功能， 蛋白质中的二硫键形成; 3.为了确定，对于那些细胞， 含有二硫键的蛋白质，当蛋白质 DsbA的作用是氧化半胱氨酸;为了分析DsbA的转运过程， 不同的半胱氨酸对在不寻常的功能中的作用 新发现的硫氧还蛋白，TrxC; 5.以确定 巯基氧化还原蛋白如硫氧还蛋白之间的特异性相互作用 及其底物。这项工作的主要组成部分涉及蛋白质 分析，虽然与DsbA和DsbB的结构功能研究 包括与实验室合作， 和这些蛋白质的生化特征。这些研究应 特别适用于工程化细菌菌株， 有效地生产包括激素在内的医用蛋白质， 抗体的过去在该项目下分离的菌株已被 用于这些有用的目的。

DESCRIPTION: (adapted from investigator's abstract) This proposal describes studies of thiol redox proteins of E. coli in terms of their function and as ways of exploring features of the process of protein translocation across the cytoplasmic membrane. The specific objectives are: 1. to use mutant analysis to examine the role of protein folding in the process of translocation of the normally cytoplasmic thioredoxin into the E. coli periplasm; 2. to use mutant analysis and biochemical studies to determine the relationship between the structure and function of two cell envelope protein, DsbA and DsbB, required for disulfide bond formation in proteins; 3. to determine, for those cell proteins that contain disulfide bonds, when during the protein translocation process the DsbA acts to oxidize cysteines; to analyze the role of different pairs of cysteines in the function of the unusual newly discovered thioredoxin, TrxC; 5. to determine the nature of the specific interactions between thiol redox proteins such as thioredoxin and its substrates. The major component of this work involves protein analysis, although the structure-function studies with DsbA and DsbB include a collaborative effort with laboratories analyzing structural and biochemical features of these proteins. These studies should be useful particularly for engineering bacterial strains that more efficiently produce proteins of medical utility including hormones and antibodies. Strains isolated under this project in the past have been used for such useful purposes.