GENES REGULATED BY ABUSED DRUGS

滥用药物通过生物化学产生长期的行为变化 这些机制在很大程度上是未知的。 药物改变 大脑中特定基因的表达可以提供一个重要的窗口， 可能的成瘾生化基质 进一步探讨 在这一领域，我们建立和发展了减微分 显示技术，允许识别几十个 对应于候选药物调节基因的不同cDNA 在大脑中表达。在本财年，我们加强了表征 其表达受安非他明调节的候选基因， 可卡因和吗啡 这些cDNA的序列分析表明， 它们分为三类：新的cDNA， 而那些属于特定基因家族的基因， 功能影响。

Abused drugs produce long-lasting changes in behaviors via biochemical mechanisms that are largely unknown. Drug-altered changes in expression of specific genes in the brain can provide a major window on possible biochemical substrates for addiction. To further explore this area, we have established and developed subtracted differential display techniques that have allowed identification of dozens of distinct cDNAs that correspond to candidate drug-regulated genes expressed in brain. During this FY, we have enhanced characterization of candidate genes whose expression is regulated by amphetamine, cocaine and morphine. Sequence analyses of these cDNAs reveal that they fall into three groups: novel cDNAs, those previously described as sequences only, and those that fall into specific gene families with functional implications.