MEMBRANE TRANSPORTERS IN MOSQUITOES

描述（改编自申请人的摘要）：该补助金的目标是 对PI的长期重点，以表征的结构和功能， 蚊子的中肠这项资助的重点是阐明 埃及伊蚊的营养吸收过程。通过分子 的方法来确定运输过程中肠，PI已分离 cDNA的3个氨基酸转运蛋白，一个潜在的蛋白质需要形成一个 具有氨基酸转运蛋白的功能性异二聚体和推定的蔗糖 埃及伊蚊中肠的转运蛋白。在目标1中，PI建议 继续分离两种蛋白质的全长cDNA克隆 他有部分cDNA这些cDNA将在异源表达载体中表达。 表达系统（非洲爪蟾卵母细胞），该系统用于表征 每种氨基酸转运蛋白的底物选择性和离子选择性 运输过程的选择性。第二个目标，类似 将对蔗糖转运蛋白进行实验。作为最终 目的分析这些转运蛋白的体内调控。的 将在体内分析基因的表达以确定是否存在 糖或血后的发育调节和调节 喂食

DESCRIPTION (Adapted From The Applicant's Abstract): This grant is targeted towards the PI's long-term focus to characterize the structure and function of the midgut of mosquitoes. This grant focuses upon elucidating the molecular processes involved in nutrient uptake in Aedes aegypti. Through a molecular approach to identify transport processes at the midgut, the PI has isolated cDNA's for 3 amino-acid transporters, a potential protein needed to form a functional heterodimer with the amino acid transporters, and a putative sucrose transporter from the midgut of Aedes aegypti. In objective 1, the PI proposes to continue in the isolation of full-length cDNA clones for two of the proteins for which he has partial cDNAs. These cDNAs will be expressed in a heterologous expression system (Xenopus oocytes) and this system used to characterize the substrate selectivity of each of the amino acid transporters and the ion selectivity of the transport process. In the second objective, similar experiments will be performed for the sucrose transporter. As the final objective, the in vivo regulation of these transporters will be analyzed. The expression of the genes will be analyzed in vivo to determine if there is developmental regulation and modulation following either sugar or blood feeding.