IMMOBILIZED LAMININ 5 FOR IMPROVED PERCUTANEOUS HEALING

经皮医疗器械，例如留置葡萄糖传感器，总是会引起过度的伤口修复反应，如上皮的激活和迁移，从而导致有袋化，有时会导致器械排斥。这些反应通常是导致设备无法正确密封并与皮肤结合的原因；并可能导致感染和设备过早失效。最近，体外实验发现外源性层粘连蛋白5（一种基底膜的关键黏附蛋白成分）可以促进非活化上皮的黏附，而不影响与伤口修复相关的增殖和迁移。因此，假设在经皮装置表面固定层粘连蛋白5可以促进其愈合。在这项工作中，层粘连蛋白5将被固定在一个模型留置葡萄糖微传感器上，使用一种新的等离子体聚合物沉积和生物分子附着方法。层粘连蛋白包被的传感器将被植入小鼠体内，并对其伤口修复反应进行组织学评估。如果成功，该项目的第二和第三阶段将研究对传感器性能的影响，固定化优化，设备对细菌挑战的抵抗力以及对商业化生产的适应性。该技术可应用于多种经皮装置。建议的商业应用：该研究有望改善留置葡萄糖传感器的愈合。该技术可用于改善许多经皮医疗器械的愈合

Percutaneous medical devices, for example an indwelling glucose sensor, invariably elicit excessive wound repair responses such as the activation and migration of epithelium, which lead to marsupilization and sometimes device exclusion. These responses are often responsible for failure of the device to properly seal and integrate with the skin; and can lead to routes for infection and premature device failure. Recently, it has been observed in vitro that exogenous Laminin 5, a key adhesive protein component of the basement membrane, can promote the adhesion of a non-activated epithelium without the proliferation and migration associated 'with wound repair. Thus, it is hypothesized that immobilization of laminin 5 on the surface of a percutaneous device may irnprove' its healing. In the proposed work, laminin 5 will be immobilized on a model indwelling glucose' microsensor, using a novel plasma polymer deposition and biomolecule attachment approach. The laminin coated sensors will be implanted in mice and the wound repair response assessed histologically. If successful, Phase II & III of the project will study the effect on sensor performance, immobilization optimization, resistance of the devices to bacterial challenge, and adaptation to commercial production. The technology may be applied to a variety of percutaneous devices. PROPOSED COMMERCIAL APPLICATION: Improved healing of an indwelling glucose sensor is expected from the research. The technologies may be applied to improve the healing of many percutaneous medical devices