MICROBIAL GENOMICS

将确定具核梭杆菌和拟杆菌基因组的完整DNA序列，并分析推定的编码区和基因产物的可能功能。这两种细菌在口腔微生物学中很重要，包括牙周炎等疾病。NIDCR的口腔传染病战略规划讲习班将它们作为DNA序列分析的目标。口腔感染性疾病通常是多微生物性质的，并且是由口腔的微生物生态的干扰引起的。从正常口腔植物群的能力以及它们之间的相互作用的角度来理解这种生态学，对于此类疾病的诊断、预防和治疗至关重要。作为第一步，阐明口腔植物群重要成员的完整遗传内容将提供丰富的信息，这些信息可以导致这些微生物的生理学、它们的毒力因子、生物膜形成机制以及它们与宿主和彼此相互作用的其他能力的深刻更深的了解。一旦基因组序列被确定和分析，它们将通过一个在线数据库提供给研究界，该数据库具有许多工具，以方便数据检索。在项目实施过程中，还将不断发布初步数据。

The complete DNA sequences of the genomes of Fusobacterium nucleatum and Bacteroides forsythus will be determined and analyzed for putative coding regions and possible functions of the gene products. These two bacteria are important in oral microbiology including diseases such as periodontitis. They have been targeted for DNA sequence analysis by a strategic planning workshop for oral infectious diseases of the NIDCR. Oral infectious diseases often are polymicrobial in nature and result from disturbances in the microbial ecology of the oral cavity. Understanding this ecology, both in terms of the capabilities of the normal oral flora as well as their interactions, is crucial to diagnosis, prevention, and cure of such diseases. As a first step, elucidating the complete genetic content of important members of the oral flora will provide a wealth of information that can lead to a profoundly deeper picture of the physiology of these microbes, their virulence factors, mechanism of biofilm formation, as well as their other capacities for interaction with the host and each other. Once the genomic sequences are determined and analyzed they will be made available to the research community through an online database with a number of tools to facilitate data retrieval. During the course of the project there will also be ongoing release of preliminary data.