MECHANISMS OF CHRONIC PROSTATITIS

描述 目前，对T的存在和作用几乎一无所知。 淋巴细胞、肥大细胞及其产物 前列腺炎（NBCP）。 因此，必须有系统地 在NBCP中对这些细胞及其产物进行表征， 填补了我们对这种炎症性疾病知识的重大空白。 因此我们 建议：1）系统地表征T淋巴细胞亚群， NBCP和非NBCP患者（关于临床标准，请参见患者人群 2）确定CD 4 Th 2相关细胞因子，特别是IL 3的作用 促进NBCP中肥大细胞的生长和分化，以及3） 确定肥大细胞及其产物在NBCP和非NBCP中的作用。 我们假设NBCP是一种前列腺炎性疾病， 是由特定T淋巴细胞亚群失衡引起的。 测试 根据这一假设，我们制定了以下具体目标：（1） 从流体中分离、表征和关联T细胞和T细胞克隆 （血液、尿液、前列腺液）和NBCP、非NBCP和正常组织 患者;（2）表征和关联CD 4 Th 1和 体液（血液，尿液， 前列腺液）和来自NBCP、非NBCP和正常患者的组织;和（3） 为了表征肥大细胞和肥大细胞的分布并将其与肥大细胞的分布相关联， NBCP、非NBCP和正常人的细胞相关因子。

DESCRIPTION Currently, virtually nothing is known about the existence and role of T lymphocytes, mast cells and their products in non bacterial chronic prostatitis (NBCP). Thus, it is essential that a systematic characterization of these cells and their products in NBCP be undertaken to fill this major gap in our knowledge of this inflammatory disease. Thus, we propose to: 1) systematically characterize T lymphocyte subpopulations in NBCP and non NBCP patients (for clinical criteria, see Patient Population below); 2) determine the role of CD4 Th2-related cytokines, particularly IL3 in promoting mast cell growth and differentiation in NBCP, and 3) to determine the role of mast cells and their products in NBCP and non NBCP. We hypothesize that NBCP is an inflammatory disease of the prostate that results from an imbalance of specific T-lymphocyte subpopulations. To test this hypothesis, we have developed the following specific aims: (1) to isolate, characterize and correlate T cells and T cell clones from fluids (blood, urine, prostate fluids) and tissue from NBCP, non NBCP and normal patient; (2) to characterize and correlate the distribution of CD4 Th1 and CD4 Th2 lymphocytes and associated cytokines in fluids (blood, urine, prostate fluids) and tissue from NBCP, non NBCP and normal patients; and (3) to characterize and correlate the distribution of mast cells and mast cell-related factors in NBCP, non NBCP and normal patients.