CHARGE TRANSFER ACROSS BIOLOGICAL INTERFACES

描述：工作的基本目标是了解这些因素 影响脂双层中的界面和跨膜电荷转移。 这项工作可能很重要，因为离子梯度的维持 跨膜是生命系统的定义品质之一。这个 这项工作的方法特点是使用光诱导转移 电子穿过脂质双分子层。这使得我们可以研究内部 静电效应。 在工作的一个阶段，建议将该方法应用于电压 门控离子通道，作为获得对通道的光控制的努力 活动。将这种方法扩展到神经细胞是可能的。 研究方向。第二条途径涉及进一步研究和 一种新型静电离子泵装置的研制 毛泽尔博士的实验室。同样，离子被驱使穿过薄膜。 通过界面上的光致电荷转移。此设备允许 准确校准跨膜现象中的静电效应。 第三个项目涉及到生物参数的确定 离子泵抽吸质子的细菌视紫红质和卤视紫红质 泵送氯离子。热焓和体积的变化与 这些光致泵浦经历了各种步骤。这些实验将 涉及光声光谱学，这是毛泽尔博士的专长。

DESCRIPTION: The basic goal of the work is to understand the factors affecting interfacial and transmembrane charge transfer in lipid bilayers. The work is potentially important, since the maintenance of ion gradients across membranes is one of the defining qualities of living systems. The approach characteristic of this work is the use of photoinduced transfer of electrons across lipid bilayers. This permits the study of internal electrostatic effects. In one phase of the work, it is proposed to apply this method to voltage gated ion channels, as an effort to gain photo-control over channel activity. The extension of the method to nerve cells is a possible direction of the research. A second avenue involves the further study and development of a device for electrostatic ion pumping that was created in Dr. Mauzerall's laboratory. Again, the ions are driven across the membrane by photoinduced charge transfer across an interface. This device allows the accurate calibration of electrostatic effects in transmembrane phenomena. The third project involves the determination of parameters of the biological ion pumps bacteriorhodopsin which pumps protons, and halorhodopsin which pumps chloride ions. The enthalpy and volume changes are associated with various steps undergone by these photoinduced pumps. These experiments will involve photoacoustic spectroscopy, a specialty of Dr. Mauzerall.