STUDIES OF MOLYBDENUM CONTAINING ENZYMES

本申请涉及四种相关的单核钼酶的基本酶学和光谱性质的综合研究：黄嘌呤氧化还原酶、亚硫酸盐氧化酶、DMSO还原酶和亚砷酸盐还原酶。 该组包括单核钼酶的所有三个主要家族的代表。 拟议工作的总体目标是通过比较和对比它们的物理，化学和电子结构，更全面地了解这些酶的作用机制的基本方面。 还将比较酶类型之间的结构与功能的关系。 为此，一系列快速反应动力学，光谱，理论和分子生物学研究将直接建立在PI实验室过去的工作。 拟议的工作是为了整合这些酶的机制和光谱研究与其已知的晶体结构，并阐明潜在的主题，通过比较和对比其性能的反应。 这种方法在研究具有血红素和黄素辅基的生物系统方面已经证明是非常成功的。

The present application involves a comprehensive investigation of the basic enzymological and spectroscopic properties of four related mononuclear molybdenum enzymes: xanthine oxidoreductase, sulfite oxidase, DMSO reductase and arsenite reductase. This group includes representatives of all three major families of mononuclear molybdenum enzymes. The overall goal of the proposed work is to gain a more complete understanding of fundamental aspects of the mechanism of action of each of these enzymes by comparing and contrasting their physical, chemical and electronic structures. A comparison among enzymes type with regard to the relationship of structure to function will also be made. To this end a series of rapid reaction kinetic, spectroscopic, theoretical and molecular biological studies will be undertaken that build directly on past work in the PI's laboratory. The proposed work is intended to integrate both mechanistic and spectroscopic studies of these enzymes with their known crystal structures and to elucidate underlying themes in reactivity by comparing and contrasting their properties. This approach that has proven extremely successful in studies of the generally much better understood biological systems that possess heme and flavin prosthetic groups.