MICHIGAN NIDDK BIOTECHNOLOGY CENTER

该提案的总体目标是在密歇根大学建立一个NIDDK生物技术中心。该中心将提供最先进的技术、设备和建议，用于测量基因表达的广泛模式。该中心将向NIDDK资助的研究人员开放，或进行与NIDDK的使命明显相关的研究。这项申请将侧重于DNA微阵列技术，这种方法非常适合密歇根大学的现有优势，资源和承诺。与此应用程序相关的资源包括物理空间，其中包含用于存储和复制EST的适当设备，用于生产和杂交微阵列芯片的设备以及用于数据分析的工具。人力资源包括经过培训的工作人员，用于执行上述步骤，包括制备标记探针和专家，为研究者提供RNA制备和数据分析方面的指导和建议。除了这种个性化的指导，NIDDK生物技术中心将通过一系列研讨会向更广泛的社区提供教育。该中心将由一个内部咨询委员会管理，该委员会包括来自三个现有NIDDK资助中心（糖尿病研究和培训中心、肾脏中心和肠道/肽中心）的代表，这些中心的成员需要获得表达分析技术。这三个中心将为NIDDK生物技术中心提供补充财政支持，医学院也是如此。内部咨询委员会将监督中心的各个方面，并将审查个人研究人员提交的获得微阵列资源的申请。两名在微阵列技术前沿享有国家声誉的外部顾问将担任顾问。该提案将受益于杰出的大学支持-该大学已承诺近200万美元用于获取和分发所有人类，大鼠和小鼠EST（序列验证），这些EST将在中央设施复制和分发给一些有针对性的研究计划，包括NIDDK生物技术中心。这些研究项目分享微阵列技术的知识和经验，并开发创新应用，包括基于寡核苷酸的新型阵列和将微阵列分析与蛋白质组学分析相结合的方法。因此，肥沃的环境将确保NIDDK生物技术中心有效地传播与表达分析相关的进展。

The overall goal of this proposal is to establish an NIDDK Biotechnology Center at the University of Michigan. The Center will provide access to state-of-the-art technology, equipment and advice for measuring broad patterns of gene expression. The Center will be accessible to investigators funded by the NIDDK or doing research clearly related to the mission of the NIDDK. This application will focus on DNA microarray technology, an approach that fits well with the existing strengths, resources and commitments of the University of Michigan. Resources connected with this application consist of physical space containing appropriate equipment for storing and replicating ESTs, equipment for producing and hybridizing microarray chips, and tools for data analysis. Personnel resources include trained staff for performing the above steps, including preparation of labeled probes and experts to provide guidance and advice to investigators in terms of RNA preparation and data analysis. In addition to such individualized instruction, the NIDDK Biotechnology Center will provide education to the broader community through a series of seminars. The Center will be governed by an Internal Advisory Committee that includes representatives from three existing NIDDK-funded Centers (Diabetes Research and Training Center, Renal Center, and Gut/'Peptide Center) whose members have needs for access to technologies for expression analysis. These three Centers will provide supplemental financial support for the NIDDK Biotechnology Center, as will the Medical School. The Internal Advisory Committee will oversee all aspects of the Center and will review applications submitted by individual investigators for access to microarray resources. Two external advisors with national reputations at the forefront of microarray technology will serve as consultants. This proposal will benefit from outstanding University support - the University has committed close to $2 million to acquire and distribute all sets of human, rat and mouse ESTs (sequence verified) available which will be replicated and distributed at a central facility to a number of targeted research programs including the NIDDK Biotechnology Center. These research programs share knowledge and experience in microarray technologies and develop innovative applications including novel oligonucleotide based arraying and means to integrate microarray analysis with proteomic analysis. Thus a fertile environment will ensure that advances made that are relevant to expression analysis will be promulgated efficiently by the NIDDK Biotechnology Center.