PURCHASE OF AN XRAY GENERATOR AND IMAGE PLATE DETECTOR

该提案要求资金购买一台配有聚焦镜的理学RU-300 X射线发生器、一台R-AXIS IV成像板系统和一台X-stream低温系统，以改善宾夕法尼亚大学医学院现有的X射线衍射设备。 最初的设备是为一个晶体学家和他的合作者开发的。 在过去的三年里，医学院已经聘请了五名新教师，他们都将使用X射线晶体学来解决大分子结构，功能和设计中的重要生物学问题。 除了聘请新的教师，大学和医学院已投资超过一百万美元，翻新7000平方英尺的结构生物学实验室空间，其中3000平方英尺将专门用于X射线晶体学。 这个新的空间将容纳现有的设备，以及本赠款中提出的新设备。仪器将由P.I.联合管理。(Dr.米奇刘易斯）和三个合作P. I.的（格雷格货车杜因博士，帕特洛尔，和希拉里纳尔逊），他们都建立了积极的晶体学计划。 博士学位1998年夏季将雇用一名高级科学家监督新空间设备的安装，并进行设备的日常维护，协助用户和培训新用户。 这位科学家也将协助其他医学院的科学家，他们不是X射线衍射专家，但希望启动晶体学项目。为了支持这一共享仪器资金的请求，生物化学和生物物理学系沿着药理学系已同意提供资金，以支持医学院新的X射线设施经理。

This proposal requests funds to purchase a Rigaku RU-300 X-ray generator with focusing mirrors, an R-AXIS IV image plate system, and an X-stream low temperature system to improve the existing X-ray diffraction facility at the University of Pennsylvania School of Medicine. The original facility was developed for a single crystallographer and his collaborators. In the past three years, the School of Medicine has hired five new faculty who will all be using X-ray crystallography to address important biological problems in macromolecular structure, function, and design. In addition to hiring new faculty, the University and the School of Medicine have invested over one million dollars to renovate 7000 square feet of laboratory space for structural biology, of which 3000 square feet will be specifically dedicated to X-ray crystallography. This new space will house the existing equipment, as well as the new equipment proposed in this grant. The instrumentation will be jointly managed by the P.I. (Dr. Mitch Lewis) and the three co- P.I.'s (Drs. Greg Van Duyne, Pat Loll, and Hillary Nelson), all of whom have established active crystallography programs. A Ph.D. level scientist will be hired during the summer of 1998 to supervise installation of the equipment in the new space, as well as to perform ongoing equipment maintenance, assist users, and train new users. This scientist will also be available to assist other Medical School scientists that are not X-ray diffraction specialists but who wish to initiate crystallographic projects. In support of this request for shared instrumentation funds, the Department of Biochemistry and Biophysics along with the Department of Pharmacology have agreed to provide funds to support the new X-ray facility manager in the Medical School.